Heat shock protein 70 / HSP70

Product Description for Heat shock protein 70 / HSP70

Mouse anti Bovine, C. elegans, Canine, Carp, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep Heat shock protein 70 / HSP70 C92F3A-5.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Immunoelectron Microscopy, Flow Cytometry.

Properties for Heat shock protein 70 / HSP70

Product Category	Antibodies
Quantity	0.2 mg
Synonyms	HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Review 003
Presentation	Aff - Purified
Reactivity	Bov, Can, Carp, Ce, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh
Applications	C, E, EM, F, ICC/IF, IP, WB
Clonality	Monoclonal
Clone	C92F3A-5
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P08107 Immunogen: Human HSP70 (1) Genename: 3303
Antibody type	Ab
Application	ELISA (10). Western blot (7,8,14). 1 µg/ml was sufficient for detection of HSP70 in 20 µg of HeLa lysate by colorimetric immunoblot analysis using HRP conjugated Goat anti-Mouse IgG as the secondary antibody. FACS (12). Immunocytochemistry (1,11). Immunoprecipitation (9). Electron Microscopy (11). Immunohistochemistry (11).
Background	Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Concentration	1.0 mg/ml
General Readings	Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. Rothman J. (1989) Cell 59: 591-601. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. Fink A.L. (1999) Physiol. Rev. 79: 425-449. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248. Dressel et al. (2000) J. Immunol. 164: 2362-2371. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer System: PBS, pH 7.2 Preservatives: 0.09% Sodium Azide Stabilizers: 50% Glycerol State: Liquid purified Ig fraction Aff - Purified
Specificity	Specificity: This antibody Detects a ~70 kDa protein corresponding to the Molecular Mass of inducible HSP70 on SDS PAGE Immunoblots. The mapped epitope is in the region of amino acid residues 436-503. Does not cross-react with HSC70 (HSP73). Species: Human, Mouse, Rat, Bovine, C. elegans, Canine, Chicken, Drosophila, Fish (carp), Guinea Pig, Hamster, Monkey, Pig, Rabbit and Sheep.
Gene ID	3303
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	Heat shock protein 70 / HSP70 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (15 products)

Catalog No.	Species	Pres.	Purity	Source
AR03018PU-L	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	0.2 mg / €710.00
					Acris Antibodies GmbH
AR03018PU-N	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03018PU-S	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	50 µg / €390.00
					Acris Antibodies GmbH
AR03022PU-L	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	0.2 mg / €710.00
					Acris Antibodies GmbH
AR03022PU-N	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03022PU-S	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	50 µg / €390.00
					Acris Antibodies GmbH
AR09035PU-L	Heat shock protein 70 / HSP70 (1-641, His-tag)
	Human	Purified	> 95 %	E. coli	0.5 mg / €540.00
					Acris Antibodies GmbH
AR09035PU-N	Heat shock protein 70 / HSP70 (1-641, His-tag)
	Human	Purified	> 95 %	E. coli	0.1 mg / €230.00
					Acris Antibodies GmbH
NBC1-18356	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18358	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18359	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18360	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18367	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
H00003304-Q01-10	Heat shock protein 70 / HSP70
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003304-Q01-25	Heat shock protein 70 / HSP70
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No.	Species
NBL1-11749	Hsp70 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat shock protein 70 / HSP70 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH