Heat shock protein 70 / HSP70

Product Description for Heat shock protein 70 / HSP70

Rabbit anti Bovine, Canine, Fish, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep, Trout Heat shock protein 70 / HSP70.
Presentation: Serum
Product is tested for Paraffin Sections, Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat shock protein 70 / HSP70

Product Category	Antibodies
Quantity	25 µl
Synonyms	HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Review 003
Presentation	Serum
Reactivity	Beluga, Bov, Can, Coral, Fi, GP, Hst, Hu, Mky, Ms, Por, Rt, Sh, Trout
Applications	C, E, ICC/IF, IP, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P08107 Immunogen: Full length Human protein HSP70 Genename: 3303
Antibody type	Ab
Application	ELISA. Western blot (7-9): 1/25,000 (ECL). Immunoprecipitation (9, 10): 1/100. Immunoflourescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.
Background	Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1, 2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
General Readings	Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. Rothman J. (1989) Cell 59: 591 -601. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. Fink A.L. (1999) Physiol. Rev. 79: 425-449. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043. Locke M. (2000) Cell Stress & Chaperones 5: 45-51. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66. Trentin G.A. et al. (2001) J Biol Chem. 276: 13087-13095. Anne Bailey and Lorne J. Hofseth. A method to enhance the sensitivity and reproducibility of immunohistochemistry http://www.prohisto.com/pdfs/Hofseth_Manuscript-81.pdf. Ings JS, Oakes KD, Vijayan MM, Servos MR. Temporal changes in stress and tissue-specific metabolic responses to municipal wastewater effluent exposure in rainbow trout. Comp Biochem Physiol C Toxicol Pharmacol. 2012 Aug;156(2):67-74. doi: 10.1016/j.cbpc.2012.04.002. Epub 2012 May 1. PubMed PMID: 22579662. Zhang D, Ciciriello F, Anjos SM, Carissimo A, Liao J, Carlile GW, et al. Ouabain Mimics Low Temperature Rescue of F508del-CFTR in Cystic Fibrosis Epithelial Cells. Front Pharmacol. 2012;3:176. doi: 10.3389/fphar.2012.00176. Epub 2012 Oct 4. PubMed PMID: 23060796.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	State: Liquid serum Serum
Specificity	Specificity: Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70 on SDS PAGE immunoblots. May crossreact with Hsc70 at lower dilutions. Species: Human, Mouse, Rat, Beluga, Cow, Dog, Fish (carp), Guinea Pig, Hamster, Monkey, Pig, Sheep, Coral, Tomato, Tobacco, Spiny dogfish shark (Squalus acanthias), Atlantic Hagfish (Myxine glutinosa), Rainbow Trout.
Gene ID	3303
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	Heat shock protein 70 / HSP70 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (15 products)

Catalog No.	Species	Pres.	Purity	Source
AR03018PU-L	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	0.2 mg / €710.00
					Acris Antibodies GmbH
AR03018PU-N	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03018PU-S	Heat shock protein 70 / HSP70 (active)
	Human	Purified	> 90 %	E. coli	50 µg / €390.00
					Acris Antibodies GmbH
AR03022PU-L	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	0.2 mg / €710.00
					Acris Antibodies GmbH
AR03022PU-N	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03022PU-S	Heat shock protein 70 / HSP70 (active)
	Human	Aff - Purified	> 95 %	E. coli	50 µg / €390.00
					Acris Antibodies GmbH
AR09035PU-L	Heat shock protein 70 / HSP70 (1-641, His-tag)
	Human	Purified	> 95 %	E. coli	0.5 mg / €540.00
					Acris Antibodies GmbH
AR09035PU-N	Heat shock protein 70 / HSP70 (1-641, His-tag)
	Human	Purified	> 95 %	E. coli	0.1 mg / €230.00
					Acris Antibodies GmbH
NBC1-18356	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18358	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18359	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18360	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18367	Heat shock protein 70 / HSP70
					0.1 mg / €280.00
					Novus Biologicals Inc.
H00003304-Q01-10	Heat shock protein 70 / HSP70
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003304-Q01-25	Heat shock protein 70 / HSP70
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No.	Species
NBL1-11749	Hsp70 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat shock protein 70 / HSP70 (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH