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NB600-1469 Heat shock protein 70 / HSP70 antibody

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50 µl / €370.00
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Quick Overview

Mouse anti Amphibian, Birds, Drosophila, Fish, Human, Mouse, Porcine, Rat, Yeast Heat shock protein 70 / HSP70 3A3

NB600-1469

Product Description for Heat shock protein 70 / HSP70

Mouse anti Amphibian, Birds, Drosophila, Fish, Human, Mouse, Porcine, Rat, Yeast Heat shock protein 70 / HSP70 3A3.
Presentation: Ascites
Product is tested for Electrophoretic Mobility Shift Assay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat shock protein 70 / HSP70

Product Category Antibodies
Quantity 50 µl
Synonyms HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Review 003
Presentation Ascites
Reactivity Amph, Av, Dros, Fi, Hu, Ms, Por, Rt, Ye
Applications EMSA, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 3A3
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P08107
Immunogen:
Human recombinant Hsp 70 over expressed in E. coli.
Genename:
3303
Antibody type Ab
Application Immunocytochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, ICC: Use at a dilution of 1/100. IHC-P: Use at an assay dependent dilution. Antigen retrieval is not essential but may optimise staining. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/5000. Predicted molecular weight: 70-78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts.Immunocytochemical staining of Hsp 70 in heat shocked HeLa cells with ab5439 results in cytoplasmic staining., immunoprecipitation , Western Blot 1/5000, Immunocytochemistry 1/100, Immunohistochemistry-Paraffin
Background The Hsp 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human Hsp 70 family members include: Hsp 70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; Hsp 72, a 72 kDa protein that is induced exclusively under stress conditions; Hsc 70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or Hsp 75, a 75 kDa protein that is found within the mitochondria.
General Readings General / background references:Inoue A et al. 70-kDa heat shock cognate protein interacts directly with the N-terminal region of the retinoblastoma gene product pRb. Identification of a novel region of pRb-mediating protein interaction. J Biol Chem 270:22571-6 (1995). Huang Q et al. Association of peroxisome proliferator-activated receptor and Hsp72. J Biol Chem 269:8493-7 (1994).
Storage Store at -20C. Avoid freeze-thaw cycles.
Format
Purification:
Ascites
Buffer System:
0.05% Sodium Azide
Purity:
Ascites
State:
Ascites
Specificity
Specificity:
Epitope mapping with a panel of Hsp 70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human Hsp 70, a region that has been shown to be involved in stress-induced nucleolar localization., NB600-1469 detects several members of the heat shock protein 70 kDa (Hsp 70) gene family including Hsp 70, Hsc 70, p75, and following heat shock, Hsp 72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.
Species:
Chicken, Drosophila, Human, Mouse, Rat, Yeast
Gene ID 3303
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (15 products)

Catalog No. Species Pres. Purity   Source  
AR03018PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03018PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03018PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR03022PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03022PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03022PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR09035PU-L

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH
AR09035PU-N

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH
NBC1-18356

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18356] - Dna K, 17.7kDa (163aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18358

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18358] - Dna K, 41.6kDa (384aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18359

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18359] - Dna K, 27.7kDa (255aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18360

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18360] - Dna K, 14.6kDa (132aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18367

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18367] - HSP70, 72.2kDa (661aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
H00003304-Q01-10

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003304-Q01-25

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-11749

Hsp70 Lysate
Western Blot: Hsp70 Lysate [NBL1-11749] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HSPA1A
0.1 mg / €370.00
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat shock protein 70 / HSP70 (8 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  
R1253AP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP
    Acris Antibodies GmbH
R1253B

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin
    Acris Antibodies GmbH
R1253F

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC
    Acris Antibodies GmbH
R1253HRP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP
    Acris Antibodies GmbH
R1253TR

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red
    Acris Antibodies GmbH
R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE
    Acris Antibodies GmbH
R1405R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    Acris Antibodies GmbH
R1455R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    Acris Antibodies GmbH
Click here to see all secondary antibodies for Heat shock protein 70 / HSP70
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