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SM5084 Heat shock protein 70 / HSP70 antibody

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0.1 ml / €370.00
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Quick Overview

Mouse anti Canine, Human, Mouse, Primate Heat shock protein 70 / HSP70 JG1

SM5084

Product Description for Heat shock protein 70 / HSP70

Mouse anti Canine, Human, Mouse, Primate Heat shock protein 70 / HSP70 JG1.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat shock protein 70 / HSP70

Product Category Antibodies
Quantity 0.1 ml
Synonyms HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Review 003
Presentation Ascites
Reactivity Can, Hu, Ms, Prim
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone JG1
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P08107
Immunogen:
Synthetic Peptide corresponding to amino acid residues 615-633 from mouse mtHSP70
AA Sequence:
G(615) S G S S G T G E Q K E D Q K E E K Q(633)
Genename:
3303
Antibody type Ab
Application Immunocytochemistry: 1/50, results in a worm-like staining pattern of mtHSP70 in DAP.3 cells, consistent with mitochondrial localization.
Immunoprecipitation.
Western blot: 1/500, detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate.
Background The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose- regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria.
mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.
General Readings
  • Hybridoma, 14(4): 347-354, 1995.
  • J. Cell Bio., 150(6): 1489-1497, 2000.
  • Mol. Cel. Bio., 23(3): 1085-1094, 2003.
Storage Aliquot and store the antibody at -20°C .
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Buffer System:
PBS containing 0.05% Sodium Azide as preservative.
State:
Liquid diluted ascites.
Ascites
Specificity
Specificity:
SM5084 detects mitochondrial heat shock protein 70 kDa (mtHSP70).
Species:
Human, Primates, Mouse, and Canine.
Gene ID 3303
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (15 products)

Catalog No. Species Pres. Purity   Source  
AR03018PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03018PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03018PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Purified > 90 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR03022PU-L

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.2 mg / €710.00
  Acris Antibodies GmbH
AR03022PU-N

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
0.1 mg / €400.00
  Acris Antibodies GmbH
AR03022PU-S

Heat shock protein 70 / HSP70 (active)
Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % E. coli
50 µg / €390.00
  Acris Antibodies GmbH
AR09035PU-L

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH
AR09035PU-N

Heat shock protein 70 / HSP70 (1-641, His-tag)
HSP70: 15% SDS PAGE Human Purified > 95 % E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH
NBC1-18356

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18356] - Dna K, 17.7kDa (163aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18358

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18358] - Dna K, 41.6kDa (384aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18359

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18359] - Dna K, 27.7kDa (255aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18360

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18360] - Dna K, 14.6kDa (132aa) with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
NBC1-18367

Heat shock protein 70 / HSP70
SDS-Page: Hsp70 Protein [NBC1-18367] - HSP70, 72.2kDa (661aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
0.1 mg / €280.00
  Novus Biologicals Inc.
H00003304-Q01-10

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00003304-Q01-25

Heat shock protein 70 / HSP70
Heat shock protein 70 / HSP70 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-11749

Hsp70 Lysate
Western Blot: Hsp70 Lysate [NBL1-11749] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HSPA1A
0.1 mg / €370.00
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat shock protein 70 / HSP70 (8 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  
R1253AP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP
    Acris Antibodies GmbH
R1253B

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    Acris Antibodies GmbH
R1253F

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC
    Acris Antibodies GmbH
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP
    Acris Antibodies GmbH
R1253TR

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red
    Acris Antibodies GmbH
R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE
    Acris Antibodies GmbH
R1405R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    Acris Antibodies GmbH
R1455R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    Acris Antibodies GmbH
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