Switch to Acris Antibodies Inc. (North America) Switch to Acris Antibodies GmbH (Europe/Worldwide)
Home » Antibodies » Heat Shock Protein 90 (HSP90) alpha/beta

AM03150PU-S Heat Shock Protein 90 (HSP90) alpha/beta antibody

See related secondary antibodies

Search for all "Heat Shock Protein 90 (HSP90) alpha/beta"

25 µg / €250.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Rat, Stapylococcus cerevisiae Heat Shock Protein 90 (HSP90) alpha/beta Hyb-K41220A

AM03150PU-S

Product Description for Heat Shock Protein 90 (HSP90) alpha/beta

Mouse anti Human, Rat, Stapylococcus cerevisiae Heat Shock Protein 90 (HSP90) alpha/beta Hyb-K41220A.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Heat Shock Protein 90 (HSP90) alpha/beta

Product Category Antibodies
Quantity 25 µg
Synonyms HSP-84, HSP-86, HSP-90, HSP84, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCA, HSPCB, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Renal carcinoma antigen NY-REN-38, Review 003
Presentation Purified
Reactivity Hu, Rt, S. cerevisiae, S.pombe
Applications C, E, WB
Clonality Monoclonal
Clone Hyb-K41220A
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P07900
Immunogen:
Recombinant Human HSP90 alpha
Genename:
3320
Antibody type Ab
Application ELISA (See Ref.1). 
Western blot: 1 µg/ml was sufficient for detection of Hsp90alpha/beta by Western Blot in 20 µg of HeLa lysate (See Ref.1,10). 
Immunohistochemistry on Frozen Sections.
Background HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of Hsp90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer.(2) From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex. (3-6) Furthermore, Hsp90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively.
Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (9).
Concentration 1.0 mg/ml
General Readings
  • Nemoto, T. et al. (1997) J.Biol Chem. 272, 26179-26187.
  • Minami, Y, et al. (1991), J.Biol Chem. 266, 10099-10103.
  • Arlander SJH, et al. (2003) J Biol Chem 278, 52572-52577.
  • Pearl H, et al. (2001) Adv Protein Chem 59,157-186.
  • Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
  • Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
  • Pratt W, Toft D. (1997) Endocr Rev 18,306–360.
  • Pratt WB. (1998) Proc Soc Exptl Biol Med 217, 420–434.
  • Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91, 8324–8328.
  • Kishimoto J, et al. (2005). Cell Stress and Chaperones. 10 (4), 296-311.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer Solution:
PBS, pH 7.2
Preservatives:
0.09% Sodium Azide
Stabilizers:
50% Glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody detects 90kD proteins corresponding to the Molecular Mass of HSP90 alpha or beta.
Species:
Human (beta-specific), Rat (Liver), S. cerevisiae, S. pombe. Limited or no cross- reactivity to rice or P. caudatum (Ref.10).
Gene ID 3320
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat Shock Protein 90 (HSP90) alpha/beta (6 products)

Catalog No. Species Pres. Purity   Source  
AR03017PU-L

HSP90AA1 / HSP90 alpha
HSP90AA1 / HSP90 alpha Human Purified > 90 % E. coli
0.2 mg / €400.00
  Acris Antibodies GmbH
AR03017PU-N

HSP90AA1 / HSP90 alpha
HSP90AA1 / HSP90 alpha Human Purified > 90 % E. coli
0.1 mg / €350.00
  Acris Antibodies GmbH
AR03017PU-S

HSP90AA1 / HSP90 alpha
HSP90AA1 / HSP90 alpha Human Purified > 90 % E. coli
50 µg / €260.00
  Acris Antibodies GmbH
SA6029

HSP90AA1 / HSP90 alpha (1-732, His-tag)
HSP90AA1 / HSP90 alpha Human Purified > 90 % E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH
SA6029X

HSP90AA1 / HSP90 alpha (1-732, His-tag)
HSP90AA1 / HSP90 alpha Human Purified > 90 % E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH
NBC1-18362

HSP90AA1 / HSP90 alpha
SDS-Page: Hsp90 Protein [NBC1-18362] - HSP90 a, 86.8kDa (752aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
0.1 mg / €390.00
  Novus Biologicals Inc.

Positive controls for Heat Shock Protein 90 (HSP90) alpha/beta (2 products)

Catalog No. Species Pres. Purity   Source  
NBL1-11743

Hsp90A Lysate
Western Blot: Hsp90A Lysate [NBL1-11743] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HSP90AA1.
0.1 mg / €500.00
  Novus Biologicals Inc.
NBL1-11744

Hsp90A Lysate
Western Blot: Hsp90A Lysate [NBL1-11744] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HSP90AA1
0.1 mg / €500.00
  Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat Shock Protein 90 (HSP90) alpha/beta (8 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  
R1253AP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP
    Acris Antibodies GmbH
R1253B

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin
    Acris Antibodies GmbH
R1253F

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC
    Acris Antibodies GmbH
R1253HRP

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP
    Acris Antibodies GmbH
R1253TR

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red
    Acris Antibodies GmbH
R1403R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE
    Acris Antibodies GmbH
R1405R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    Acris Antibodies GmbH
R1455R

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    Acris Antibodies GmbH
Click here to see all secondary antibodies for Heat Shock Protein 90 (HSP90) alpha/beta
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

DIN-EN-ISO-9001
Acris Antibodies Newsletter
PTG money back guarantee
Link-A-Light Conjugation Kits
Protein Biotechnologies - Ready to use lysates, cell lines, tissue microarrays and more...
Antibody Location Service

Antibody Location Service

Over 230,000 products but you can't find the right antibody for your protein or application?
Acris will do the search for you!

Free Antibody Location Service
Service
 
© Copyright 1998 - 2013 Acris Antibodies GmbH