Heat Shock Protein 90 / HSP90 (alpha + beta) - NB100-1972

Product Description for Heat Shock Protein 90 / HSP90

Mouse anti Bacteria, Bovine, Canine, Chicken, Fish, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep Heat Shock Protein 90 / HSP90 AC88.
Properties: (alpha + beta)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Heat Shock Protein 90 / HSP90

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	HSP-84, HSP-86, HSP-90, HSP84, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCA, HSPCB, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Renal carcinoma antigen NY-REN-38, Review 003
Presentation	Purified
Reactivity	Bac, Bov, Can, Chk, Fi, GP, Hu, Mky, Ms, Por, Rb, Rt, Sh
Applications	P, WB
Clonality	Monoclonal
Clone	AC88
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P08238 Immunogen: Achlya ambisexualis (water mold) Hsp90. Genename: 3326
Property	alpha + beta
Antibody type	Ab
Application	flow cytometry / FACS analysis, Immunocytochemistry, immunohistochemistry, immunoprecipitation, Western Blot, When used in immunoprecipitation, this antibody does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus. WB: Detects a band of approximately 90 kDa (predicted molecular weight: 84.7 kDa(alpha) and 83.2 kDa (beta)). Optimal dilutions/concentrations should be determined by the end user., flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation 4 ug/ml, Western Blot 1 ug/ml, Immunocytochemistry ,
Background	In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
Concentration	1 mg/ml
General Readings	General / background references:Feng H et al. Stressed apoptotic tumor cells stimulate dendritic cells and induce specific cytotoxic T cells. Blood 100:4108-15 (2002). Gebhard B et al. MHC-class-I expression in human breast cancer correlates with nuclear localization of the 90 kDa heat-shock-protein. Anticancer Res 19:5293-7 (1999). Imamura T et al. Involvement of heat shock protein 90 in the degradation of mutant insulin receptors by the proteasome. J Biol Chem 273:11183-8 (1998). Smith DF et al. Identification of a 60-kilodalton stress-related protein, p60, which interacts with hsp90 and hsp70. Mol Cell Biol 13:869-76 (1993). Redmond T et al. Immunofluorescence colocalization of the 90-kDa heat-shock protein and microtubules in interphase and mitotic mammalian cells. Eur J Cell Biol 50:66-75 (1989). Riehl RM et al. Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species. Biochemistry 24:6586-91 (1985).
Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format	Purification: protein G purified Buffer System: No Preservative Purity: protein G purified State: Purified
Specificity	Specificity: The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence., Recognizes both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa. Species: Bovine, Canine, Chicken, Fish, Guinea Pig, Hamster, Human, Mouse, Primate, Rabbit, Rat, Sheep
Gene ID	3326
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	HSP90AB1 / HSP90 beta antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat Shock Protein 90 / HSP90 (2 products)

Catalog No.	Species	Pres.
H00003326-P01-10	HSP90AB1 / HSP90 beta
	Human	Purified	10 µg / €350.00
			Abnova Taiwan Corp.
H00003326-P01-25	HSP90AB1 / HSP90 beta
	Human	Purified	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for Heat Shock Protein 90 / HSP90 (1 products)

Catalog No.	Species
NBL1-11745	Hsp90B Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat Shock Protein 90 / HSP90 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH