Heat Shock Protein 90 / HSP90 (alpha + beta) - SM5069

Product Description for Heat Shock Protein 90 / HSP90

Mouse anti Chicken, Fish, Human, Mouse, Rat Heat Shock Protein 90 / HSP90 3G3.
Properties: (alpha + beta)
Presentation: Ascites
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat Shock Protein 90 / HSP90

Product Category	Antibodies
Quantity	0.4 ml
Synonyms	HSP-84, HSP-86, HSP-90, HSP84, HSP86, HSP90A, HSP90AA1, HSP90AB1, HSP90B, HSPC1, HSPC2, HSPCA, HSPCB, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90-beta, Renal carcinoma antigen NY-REN-38, Review 003
Presentation	Ascites
Reactivity	Chk, Fi, Hu, Ms, Rt
Applications	IP, WB
Clonality	Monoclonal
Clone	3G3
Host	Mouse
Isotype	IgM
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P07900 Immunogen: Purified Mouse HSP90 from Hepa 1 cytosol. Genename: 3320
Property	alpha + beta
Antibody type	Ab
Add. information	Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. Resuspend pellet in 200µl of PBS, pH 7.2. If desired, 0.05% Sodium Azide may be added to resuspended solution. Removal of residual Ammonium Sulfate is not usually necessary for Immunoprecipitation and Western blot procedures, however, any other uses may require its removal by dialysis or equivalent method. Stable for 6 months after PBS resuspension.
Application	Immunoprecipitation: It has been reported that up to 90% of cytosolic HSP90 can be immunoprecipitated with this product under low ionic strength conditions. Western Blot (1/500): Detects a 90 kDa protein representing HSP90 from Hepa 1 cell extract. Nitrocellulose is recommended. PVDF membranes typically do not yield acceptable results with this antibody.
Background	Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
Concentration	0.575 ng/ml
General Readings	FEBS 331(1,2):25-30, 1993. J. Biol. Chem. 264(26): 15542-15547, 1989. J. Biol. Chem. 266: 6708-6713, 1991. JBC Vol 279, No 47, 49307-49314, Nov 2004. Mol. Endocrinology doi:10.1210/me.2004-0264, Jan. 2005.
Storage	Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. State: Ammonium Sulfate/Ascites Suspension without preservatives. Ascites
Specificity	Specificity: SM5069 detects Heat Sshock Protein 90 kDa (HSP90) from Human, Mouse, Rat, Rainbow trout, and Chicken tissues. Species: Non-human primate, Human, Rat, Mouse, Fish and Chicken.
Gene ID	3320
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	HSP90AA1 / HSP90 alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat Shock Protein 90 / HSP90 (6 products)

Catalog No.	Species	Pres.	Purity	Source
AR03017PU-L	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	0.2 mg / €400.00
					Acris Antibodies GmbH
AR03017PU-N	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	0.1 mg / €350.00
					Acris Antibodies GmbH
AR03017PU-S	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	50 µg / €260.00
					Acris Antibodies GmbH
SA6029	HSP90AA1 / HSP90 alpha (1-732, His-tag)
	Human	Purified	> 90 %	E. coli	0.1 mg / €370.00
					Acris Antibodies GmbH
SA6029X	HSP90AA1 / HSP90 alpha (1-732, His-tag)
	Human	Purified	> 90 %	E. coli	0.5 mg / €990.00
					Acris Antibodies GmbH
NBC1-18362	HSP90AA1 / HSP90 alpha
					0.1 mg / €390.00
					Novus Biologicals Inc.

Positive controls for Heat Shock Protein 90 / HSP90 (2 products)

Catalog No.	Species
NBL1-11743	Hsp90A Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.
NBL1-11744	Hsp90A Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for Heat Shock Protein 90 / HSP90 (9 products)

Catalog No.	Host	Pres.
R1349AP	Mouse IgA (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1349B	Mouse IgA (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1349F	Mouse IgA (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1349HRP	Mouse IgA antibody
	Goat	HRP
			Acris Antibodies GmbH
R1349T	Mouse IgA (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1349TR	Mouse IgA (H+L chain) antibody
	Goat	Texas Red
			Acris Antibodies GmbH
SP1018F	Mouse IgM (Mu chain specific), adsorbed antibody
	Goat	FITC
			Acris Antibodies GmbH
SP1018HRP	Mouse IgM (Mu chain specific), adsorbed antibody
	Goat	HRP
			Acris Antibodies GmbH
SP1018R	Mouse IgM (Mu chain specific), adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH