HIF1A / HIF1 alpha

Product Description for HIF1A / HIF1 alpha

Mouse anti Bovine, Canine, Human, Mouse, Rat HIF1A / HIF1 alpha ESEE122.
Presentation: Ascites
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for HIF1A / HIF1 alpha

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Presentation	Ascites
Reactivity	Bov, Can, Hu, Ms, Rt
Applications	C, ICC/IF, IP, P, WB
Clonality	Monoclonal
Clone	ESEE122
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: Q16665 Immunogen: Human HIF-1 alpha amino acids 329-530. Genename: 3091
Antibody type	Ab
Add. information	Research Areas: Cancer, Hypoxia
Application	Immunocytochemistry, immunofluorescence, immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Works well on most histochemical applications. NOT recommended specifically for Western analysis, although some people have used it and prefer it for Western Blot. Procedure: http://ihcworld.com/_protocols/antibody_protocols/hif_1alpha_novus.htm, immunohistochemistry 1:100-1:5000, immunofluorescence , immunoprecipitation , Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen ,
Background	Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Concentration	1 mg/ml
General Readings	These references discuss the use of this clone but not this particular antibody production. 1. Talks, K.L. and Turley, H. Amer. J. of Pathology. 157: 411-421 (2000) (Immunohistochemistry and Western Blot) 2. Weisener, M.S. and Turley, H. Blood. 92: 2260-2268 (1998) 3. Giatromanolaki, A., et al. Cancer Research. 61: 7992-7998 (2001) 4. Koukourakis, MI, et al. Cancer Research. 61: 1830-1832 (2001) (Immunohistochemistry) 5. Beasley, Nigel J.P., et al. Cancer Research. 62: 2493-2497 (2002) (Immunohistochemistry) 6. Choke, E., Cockerill, G. W., Dawson, J., Chung, Y.-L., Griffiths, J., Wilson, R. W., Loftus, I. M., and Thompson, M. M. (2006) Hypoxia at the Site of Abdominal Aortic Aneurysm Rupture Is Not Associated with Increased Lactate. 1085: 306-310. 7. Khan, Z., Michalopoulos, G. K., and Stolz, D. B. (2006) Peroxisomal Localization of Hypoxia-Inducible Factors and Hypoxia-Inducible Factor Regulatory Hydroxylases in Primary Rat Hepatocytes Exposed to Hypoxia-Reoxygenation, 169, 1251-1269.
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: protein G purified Buffer System: 0.01% Sodium Azide Purity: protein G purified State: Ascites
Specificity	Specificity: This is specific for HIF-1 alpha. Species: Bovine, Human, Mouse, Rat
Gene ID	3091
Targets	HIF1A / HIF1 alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for HIF1A / HIF1 alpha (14 products)

Catalog No.	Species	Pres.	Purity	Source
AR09585PU-L	HIF1A / HIF1 alpha (576-785, His-tag)
	Human	Purified	> 85 %	E. coli	0.25 mg / €750.00
					Acris Antibodies GmbH
AR09585PU-N	HIF1A / HIF1 alpha (576-785, His-tag)
	Human	Purified	> 85 %	E. coli	50 µg / €300.00
					Acris Antibodies GmbH
AR09668PU-N	HIF1A / HIF1 alpha (1-85, His-tag)
	Human	Purified	> 80 %	E. coli	50 µg / €750.00
					Acris Antibodies GmbH
AR09668PU-S	HIF1A / HIF1 alpha (1-85, His-tag)
	Human	Purified	> 80 %	E. coli	10 µg / €300.00
					Acris Antibodies GmbH
AR39117PU-L	HIF1A / HIF1 alpha (530-826)
	Human	Purified	> 95 %	E. coli	0.5 mg / €540.00
					Acris Antibodies GmbH
AR39117PU-N	HIF1A / HIF1 alpha (530-826)
	Human	Purified	> 95 %	E. coli	0.1 mg / €230.00
					Acris Antibodies GmbH
NBC1-18422	HIF1A / HIF1 alpha
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBP1-45300-0.25	HIF1A / HIF1 alpha
	Human	Purified			0.25 mg / €740.00
					Novus Biologicals Inc.
NBP1-45300-50	HIF1A / HIF1 alpha
	Human	Purified			50 µg / €340.00
					Novus Biologicals Inc.
NBP1-49165-10	HIF1A / HIF1 alpha
	Human	Purified			10 µg / €340.00
					Novus Biologicals Inc.
NBP1-49165-50	HIF1A / HIF1 alpha
	Human	Purified			50 µg / €740.00
					Novus Biologicals Inc.
H00003091-P01	HIF1A / HIF1 alpha
	Human	Purified			2 µg / €250.00
					Abnova Taiwan Corp.
H00003091-Q01-10	HIF1A / HIF1 alpha
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003091-Q01-25	HIF1A / HIF1 alpha
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for HIF1A / HIF1 alpha (1 products)

Catalog No.	Species
H00003091-T01	HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for HIF1A / HIF1 alpha (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH