HIF1A / HIF1 alpha

Product Description for HIF1A / HIF1 alpha

Rabbit anti Mouse HIF1A / HIF1 alpha.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for HIF1A / HIF1 alpha

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Presentation	Aff - Purified
Reactivity	Ms
Applications	C, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: Q16665 Immunogen: A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein. Genename: 3091
Antibody type	Ab
Add. information	Research Areas: Hypoxia, Cancer
Application	immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, This antibody has been tested in Western blot, recognizing a band in hypoxic samples at ~115 kDa. It has only been tested against mouse, rat, human and primate protein. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. This antibody is not recommended for immunoprecipitation. It has also been used for immunohistochemistry (see procedure guide)., immunohistochemistry 5-10 ug/ml, Western Blot 1:500-1:2000, Immunohistochemistry-Paraffin 5-10 ug/ml, 000
Background	Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Concentration	1 mg/ml
Storage	Store at 4°C. Do Not Freeze.
Format	Purification: Immunogen affinity purified Preservatives: 0.05% Sodium Azide Purity: Immunogen affinity purified State: Aff - Purified
Specificity	Specificity: This is specific for mouse HIF-1 alpha. Species: Human, Mouse, Primate, Rat
Gene ID	3091
Targets	HIF1A / HIF1 alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for HIF1A / HIF1 alpha (14 products)

Catalog No.	Species	Pres.	Purity	Source
AR09585PU-L	HIF1A / HIF1 alpha (576-785, His-tag)
	Human	Purified	> 85 %	E. coli	0.25 mg / €750.00
					Acris Antibodies GmbH
AR09585PU-N	HIF1A / HIF1 alpha (576-785, His-tag)
	Human	Purified	> 85 %	E. coli	50 µg / €300.00
					Acris Antibodies GmbH
AR09668PU-N	HIF1A / HIF1 alpha (1-85, His-tag)
	Human	Purified	> 80 %	E. coli	50 µg / €750.00
					Acris Antibodies GmbH
AR09668PU-S	HIF1A / HIF1 alpha (1-85, His-tag)
	Human	Purified	> 80 %	E. coli	10 µg / €300.00
					Acris Antibodies GmbH
AR39117PU-L	HIF1A / HIF1 alpha (530-826)
	Human	Purified	> 95 %	E. coli	0.5 mg / €540.00
					Acris Antibodies GmbH
AR39117PU-N	HIF1A / HIF1 alpha (530-826)
	Human	Purified	> 95 %	E. coli	0.1 mg / €230.00
					Acris Antibodies GmbH
NBC1-18422	HIF1A / HIF1 alpha
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBP1-45300-0.25	HIF1A / HIF1 alpha
	Human	Purified			0.25 mg / €740.00
					Novus Biologicals Inc.
NBP1-45300-50	HIF1A / HIF1 alpha
	Human	Purified			50 µg / €340.00
					Novus Biologicals Inc.
NBP1-49165-10	HIF1A / HIF1 alpha
	Human	Purified			10 µg / €340.00
					Novus Biologicals Inc.
NBP1-49165-50	HIF1A / HIF1 alpha
	Human	Purified			50 µg / €740.00
					Novus Biologicals Inc.
H00003091-P01	HIF1A / HIF1 alpha
	Human	Purified			2 µg / €250.00
					Abnova Taiwan Corp.
H00003091-Q01-10	HIF1A / HIF1 alpha
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003091-Q01-25	HIF1A / HIF1 alpha
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for HIF1A / HIF1 alpha (1 products)

Catalog No.	Species
H00003091-T01	HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for HIF1A / HIF1 alpha (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH