HSP60

Product Description for HSP60

Mouse anti Chicken, Human, Mouse, Rat HSP60 LK1.
Presentation: Purified
Product is tested for Immunoelectron Microscopy, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for HSP60

Product Category	Antibodies
Quantity	0.5 ml
Synonyms	60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, CPN60, Chaperonin 60, GROEL, GroEL Homolog, HSP-60, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Review 003
Presentation	Purified
Reactivity	Chk, Hu, Ms, Rt
Applications	EM, ICC/IF, IP, P, WB
Clonality	Monoclonal
Clone	LK1
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P10809 Immunogen: Recombinant full length protein. Genename: 3329
Antibody type	Ab
Application	immunofluorescence, immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunomicroscopy, immunoprecipitation, Western Blot, Electron microscopy, immunohistochemistry, immunofluorescence, immunoprecipitation, Immunohistochemistry-Paraffin and frozen and Western Blot: Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user. IHC: Ab 1:50 for 30 min at RT IF: assay dependent IP: Use Protein G; Ab at 2ug/mg protein lysate WB: Ab 1-2ug/ml for 2hrs ar RT, immunohistochemistry 1:50, immunofluorescence, immunoprecipitation 1-2ug, Western Blot 1-2ug, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:50, Immunomicroscopy
Background	Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.
Concentration	0.2 mg/ml
General Readings	This product has been used in:Boog CJ et al. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. J Exp Med 175:1805-10 (1992).
Storage	Store at 4C. Do not freeze.
Format	Purification: protein G purified Buffer System: 0.09% Sodium Azide Purity: protein G purified State: Purified
Specificity	Specificity: This recognizes only the mammalian (not bacterial) Hsp 60 and is useful indistinguishing Hsp 60 from mammals and bacteria. Species: Chicken, Hamster, Human, Mouse, Rat
Gene ID	3329
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	HSP60 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for HSP60 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR03019PU-L	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	0.2 mg / €590.00
					Acris Antibodies GmbH
AR03019PU-N	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03019PU-S	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	50 µg / €310.00
					Acris Antibodies GmbH
NBC1-18353	HSP60
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18372	HSP60
					0.1 mg / €340.00
					Novus Biologicals Inc.
H00003329-P01-10	HSP60
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003329-P01-25	HSP60
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for HSP60 (3 products)

Catalog No.	Species
NBL1-11766	Hsp60 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
NBL1-11767	Hsp60 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00003329-T01	HSPD1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for HSP60 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH