HSP60

Product Description for HSP60

Mouse anti Human, Mouse, Rat HSP60 SJ-60.
Product is tested for Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for HSP60

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, CPN60, Chaperonin 60, GROEL, GroEL Homolog, HSP-60, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Review 003
Reactivity	Hu, Ms, Rt
Applications	IP, P, WB
Clonality	Monoclonal
Clone	SJ-60
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P10809 Immunogen: Recombinant human heat shock protein 60 (HSP60)1, Genename: 3329
Antibody type	Ab
Add. information	,
Application	This antibody is useful in Western Blot, Immunohistochemistry, Immunoprecipitation., Western Blot 2-4ul/ml, Immunohistochemistry-Paraffin 4-8ul/ml.
Background	In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the singlering human mitochondrial homolog, Hsp60 with its cochaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
General Readings	,
Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format	Purification: IgG purified Buffer System: PBS
Specificity	Species: Human, Mouse, Rat
Gene ID	3329
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	HSP60 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for HSP60 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR03019PU-L	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	0.2 mg / €590.00
					Acris Antibodies GmbH
AR03019PU-N	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	0.1 mg / €400.00
					Acris Antibodies GmbH
AR03019PU-S	HSP60 (active)
	Human	Aff - Purified	> 90 %	E. coli	50 µg / €310.00
					Acris Antibodies GmbH
NBC1-18353	HSP60
					0.1 mg / €280.00
					Novus Biologicals Inc.
NBC1-18372	HSP60
					0.1 mg / €340.00
					Novus Biologicals Inc.
H00003329-P01-10	HSP60
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00003329-P01-25	HSP60
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for HSP60 (3 products)

Catalog No.	Species
NBL1-11766	Hsp60 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
NBL1-11767	Hsp60 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00003329-T01	HSPD1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for HSP60 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH