HSP90AA1 / HSP90 alpha

Product Description for HSP90AA1 / HSP90 alpha

Mouse anti Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat HSP90AA1 / HSP90 alpha D7alpha.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for HSP90AA1 / HSP90 alpha

Product Category	Antibodies
Quantity	25 µg
Synonyms	HSP86, HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, NY-REN-38, Review 003
Presentation	Purified
Reactivity	Bov, Chk, Hu, Ms, Por, Rb, Rt
Applications	C, IP, WB
Clonality	Monoclonal
Clone	D7alpha
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P07900 Immunogen: Full length protein purified from chicken brain, clone D7α
Antibody type	Ab
Application	Immunoprecipitation (1, 2, 3): 5 µg with 20 µl Protein A beads. Immunohistochemistry on frozen sections. Western blot: 1:500.
Background	Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immune-oadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (10).
Concentration	1.0 mg/ml
General Readings	Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296. Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880. Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328.
Storage	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: Mouse IgG in PBS buffer, 0.09 % sodium azide and 50 % glycerol State: Liquid purified Ig fraction Purified
Species Reactivity	Species reactivity (tested): Bovine, Chicken, Human, Mouse, Porcine (Pig), Rat, Rabbit.
Specificity	Specificity: This antibody recognizes 90kDa proteins corresponding to the molecular mass of hsp90. Hsp90alpha specific for human samples. Can isolate complexes of hsp90, Src kinase and cdc37 (1, 2, 3).
FocusOn and Reviews	Reviews: Antibodies to Heat Shock Proteins - Product Review 03
Targets	HSP90AA1 / HSP90 alpha antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for HSP90AA1 / HSP90 alpha (6 products)

Catalog No.	Species	Pres.	Purity	Source
AR03017PU-L	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	0.2 mg / €400.00
					Acris Antibodies GmbH
AR03017PU-N	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	0.1 mg / €350.00
					Acris Antibodies GmbH
AR03017PU-S	HSP90AA1 / HSP90 alpha
	Human	Purified	> 90 %	E. coli	50 µg / €260.00
					Acris Antibodies GmbH
SA6029	HSP90AA1 / HSP90 alpha (1-732, His-tag)
	Human	Purified	> 90 %	E. coli	0.1 mg / €370.00
					Acris Antibodies GmbH
SA6029X	HSP90AA1 / HSP90 alpha (1-732, His-tag)
	Human	Purified	> 90 %	E. coli	0.5 mg / €990.00
					Acris Antibodies GmbH
NBC1-18362	HSP90AA1 / HSP90 alpha
					0.1 mg / €390.00
					Novus Biologicals Inc.

Positive controls for HSP90AA1 / HSP90 alpha (2 products)

Catalog No.	Species
NBL1-11743	Hsp90A Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.
NBL1-11744	Hsp90A Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for HSP90AA1 / HSP90 alpha (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH