IFNA1 / Interferon-alpha 1 - SM096PS

Product Description for IFNA1 / Interferon-alpha 1

Rat anti Mouse IFNA1 / Interferon-alpha 1 F18.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Functional assay, Flow Cytometry.

Properties for IFNA1 / Interferon-alpha 1

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	IFN-alpha 1, IFNA13, Interferon alpha-D, LeIF D
Presentation	Purified
Reactivity	Ms
Applications	E, F, FN
Clonality	Monoclonal
Clone	F18
Host	Rat
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P01572 Genename: 15962
Antibody type	Ab
Application	Flow cytometry (2): For intracellular staining of IFN-α, cells can be fixed in 1 % formaldehyde; blocked and permeabilized in 0.2 % saponin, 5 % normal rabbit serum for 30 minutes on ice (Ref.2). The typical starting working dilution is 1:10. Functional assays (3): Neutralization of IFN-α by adding 1 µg antibody F18 per mouse i.v. before treatment with 35 µg LPS i.p., decreased the LPS-induced IL-1β serum response (Ref.3). Immunoassays (1). Positive control: Plasmacytoid dendritic cells.
Background	The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. Interferon alpha is produced primarily by plasmacytoid dendritic cells and is a potent component of the anti viral innate immune response; it modulates adaptive immunity. The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
Concentration	0.1 mg/ml
General Readings	Dalod M, Salazar-Mather TP, Malmgaard L, Lewis C, Asselin-Paturel C, Brière F, et al. Interferon alpha/beta and interleukin 12 responses to viral infections: pathways regulating dendritic cell cytokine expression in vivo. J Exp Med. 2002 Feb 18;195(4):517-28. PubMed PMID: 11854364. Diebold SS, Montoya M, Unger H, Alexopoulou L, Roy P, Haswell LE, et al. Viral infection switches non-plasmacytoid dendritic cells into high interferon producers. Nature. 2003 Jul 17;424(6946):324-8. Epub 2003 Jun 22. PubMed PMID: 12819664. Joshi VD, Kalvakolanu DV, Chen W, Zhang L, Kang TJ, Thomas KE, et al. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression. J Interferon Cytokine Res. 2006 Oct;26(10):739-47. PubMed PMID: 17032168.
Storage	Store at 2 - 8 °C. Shelf life: one year from despatch.
Format	Purification: Protein G Buffer System: PBS Stabilizers: 0.1% bovine serum albumin State: Liquid 0.2 µm filtered Ig fraction Purified
Species Reactivity	Species reactivity (tested): Mouse
Specificity	Specificity: This antibody recognises most isoforms of natural and recombinant alpha Interferon. It does not cross-react with Murine beta or gamma Interferon, nor with Human alpha Interferon. The antibody exhibits neutralizing activity of approximately 6.25 x 10e4 neutralizing units/mg.
Gene ID	15962
Targets	IFNA1 / Interferon-alpha 1 antibody

Accessory Products

Recommended Secondary Antibodies for IFNA1 / Interferon-alpha 1 (8 products)

Catalog No.	Host	Pres.
R1371AP	Rat IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1371B	Rat IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1371F	Rat IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1371HRP	Rat IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1371T	Rat IgG (H+L chain) antibody
	Rabbit	TRITC
			Acris Antibodies GmbH
R1413R	Rat IgG (H+L chain), F(ab)2 Fragment antibody
	Goat	PE
			Acris Antibodies GmbH
R1415R	Rat IgG F(ab)2 specific (adsorbed F(ab)2 Fragment) antibody
	Goat	PE
			Acris Antibodies GmbH
R1428R	Rat IgG (H+L chain) F(ab')2 Fragment antibody
	Donkey	PE
			Acris Antibodies GmbH