Product Description for Ki-67
Rabbit anti Human, Rat Ki-67 SP6.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Western blot / Immunoblot, Paraffin Sections.
Properties: (C-term)
Presentation: Supernatant
Product is tested for Western blot / Immunoblot, Paraffin Sections.
Properties for Ki-67
| Product Category | Antibodies |
|---|---|
| Quantity | 0.5 ml |
| Synonyms | FocusOn056, Ki67 antigen, MKI67, Proliferation marker |
| Presentation | Supernatant |
| Reactivity | Hu, Rt |
| Applications | P, WB |
| Clonality | Monoclonal |
| Clone | SP6 |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | C-term |
| Antibody type | Ab |
| Application | Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/100 dilution in an ABC method for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Also suitable for Western blot. |
| Background | Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation. |
| Storage | Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format |
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| Species Reactivity |
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| Specificity |
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| Gene ID | 4288 |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Ki-67 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00004288-Q01-10 |
Ki-67 |
||||||
 |
Human | in vitro transl. |
10 µg /
€350.00
|
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| Abnova Taiwan Corp. | |||||||
| H00004288-Q01-25 |
Ki-67 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Ki-67 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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