BP4505S Lamin-A/C (LMNA) (C Isoform specific) antibody

Swiss Prot Num:

P02545

Immunogen:

A peptide made from the last 8 amino acids of Lamin C, including an N-terminal lysine as a linker (KHHVSGSRR), coupled to KLH

Genename:

4000

• Venables RS, McLean S, Luny D, Moteleb E, Morley S, Quinlan RA, et al. Expression of individual lamins in basal cell carcinomas of the skin. Br J Cancer. 2001 Feb;84(4):512-9. PubMed PMID: 11207047.
• Tilli, C. M., Ramaekers, F. C., Broers, J. L., Hutchison, C. J., and Neumann, H. A. (2003). Lamin expression in normal human skin, actinic keratosis, squamous cell carcinoma and basal cell carcinoma. Br J Dermatol 148, 102-109. PMID: 11207047 
• Broers JL, Bronnenberg NM, Kuijpers HJ, Schutte B, Hutchison CJ, Ramaekers FC. Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis. Eur J Cell Biol. 2002 Dec;81(12):677-91. PubMed PMID: 12553668.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Purification:

Affinity Chromatography

Buffer System:

PBS

Preservatives:

0.09% Sodium Azide

State:

Liquid purified Ig fraction
Aff - Purified

Species reactivity (tested):

Human, Mouse and Rat.

Specificity:

This antibody reacts exclusively with Lamin C.
It reacts strongly in Immunoblotting with fragments corresponding to peptide 319-572 aa of Lamin C. When tested against nuclear protein extracts from HeLa cells it reacts with a single band migrating at 65 kDa.