Lamin-B1 (LMNB1)

Product Description for Lamin-B1 (LMNB1)

Mouse anti Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish Lamin-B1 (LMNB1) 119D5-F1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry.

Properties for Lamin-B1 (LMNB1)

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	FocusOn028, FocusOn098, LMN2, LMNB, LMNB-1, LMNB1, Lamin-B1, Nuclear Envelope Marker
Presentation	Purified
Reactivity	Bov, Can, Hu, Ms, Rb, Rt, Sh, Ze
Applications	C, E, F, ICC/IF, WB
Clonality	Monoclonal
Clone	119D5-F1
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P20700 Immunogen: Purified Rat liver lamins. Genename: 4001
Antibody type	Ab
Application	ELISA. Flow Cytometry: 1/100-1/200. Immunocytochemistry: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Immunoblotting: 1/100-1/1000.
Background	Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
Concentration	1.0 mg/ml
General Readings	Weaver VM, Carson CE, Walker PR, Chaly N, Lach B, Raymond Y, et al. Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes. J Cell Sci. 1996 Jan;109 ( Pt 1):45-56. PubMed PMID: 8834789. Pugh GE, Coates PJ, Lane EB, Raymond Y, Quinlan RA. Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells. J Cell Sci. 1997 Oct;110 ( Pt 19):2483-93. PubMed PMID: 9410886. Broers JL, Machiels BM, Kuijpers HJ, Smedts F, van den Kieboom R, Raymond Y, et al. A- and B-type lamins are differentially expressed in normal human tissues. Histochem Cell Biol. 1997 Jun;107(6):505-17. PubMed PMID: 9243284. Machiels BM, Broers JL, Raymond Y, de Ley L, Kuijpers HJ, Caberg NE, et al. Abnormal A-type lamin organization in a human lung carcinoma cell line. Eur J Cell Biol. 1995 Aug;67(4):328-35. PubMed PMID: 8521872. Machiels BM, Ramaekers FC, Kuijpers HJ, Groenewoud JS, Oosterhuis JW, Looijenga LH. Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults. J Pathol. 1997 Jun;182(2):197-204. PubMed PMID: 9274531. Jansen MP, Machiels BM, Hopman AH, Broers JL, Bot FJ, Arends JW, et al. Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease. Histopathology. 1997 Oct;31(4):304-12. PubMed PMID: 9363444. Neri LM, Raymond Y, Giordano A, Capitani S, Martelli AM. Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells. J Cell Physiol. 1999 Mar;178(3):284-95. PubMed PMID: 9989774. Broers JL, Bronnenberg NM, Kuijpers HJ, Schutte B, Hutchison CJ, Ramaekers FC. Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis. Eur J Cell Biol. 2002 Dec;81(12):677-91. PubMed PMID: 12553668.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: PBS containing 0.09% Sodium Azide as preservative State: Liquid purified Ig fraction Purified
Specificity	Specificity: The antibody reacts with an epitope located C-terminal of residue 231 in lamin B1. Species: Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish.
Gene ID	4001
FocusOn and Reviews	FocusOns: Antibodies to Lamins - FocusOn 028 Organelle Markers: Antibodies to the Nucleus - FocusOn 098
Targets	Lamin-B1 (LMNB1) antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Lamin-B1 (LMNB1) (4 products)

Catalog No.	Species	Pres.
H00004001-P01-10	Lamin-B1 (LMNB1)
	Human	Purified	10 µg / €350.00
			Abnova Taiwan Corp.
H00004001-P01-25	Lamin-B1 (LMNB1)
	Human	Purified	25 µg / €500.00
			Abnova Taiwan Corp.
H00004001-Q01-10	Lamin-B1 (LMNB1)
	Human	Purified	10 µg / €350.00
			Abnova Taiwan Corp.
H00004001-Q01-25	Lamin-B1 (LMNB1)
	Human	Purified	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for Lamin-B1 (LMNB1) (1 products)

Catalog No.	Species
H00004001-T01	LMNB1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Lamin-B1 (LMNB1) (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH