Lamin-B2 (LMNB2) - BM4502 | acris-antibodies.com

Product Description for Lamin-B2 (LMNB2)

Mouse anti Hamster, Human, Mouse, Porcine, Xenopus, Zebrafish Lamin-B2 (LMNB2) LN43.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for Lamin-B2 (LMNB2)

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	FocusOn028, FocusOn098, LMN2, LMNB, LMNB-2, LMNB2, Lamin-B2, Nuclear Envelope Marker
Presentation	Purified
Reactivity	Hst, Hu, Ms, Por, Xen, Ze
Applications	C, F, WB
Clonality	Monoclonal
Clone	LN43
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q03252 Immunogen: Detergent insoluble fraction of potoroo cell line PtK1. Genename: 84823
Antibody type	Ab
Application	Suitable for Immunocytochemistry on Frozen Sections, Immunoblotting and Flow Cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. 1/100-1/1000 for Immunoblotting applications.
Background	An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.
Concentration	1.0 mg/ml
General Readings	Bridger JM, Kill IR, O'Farrell M, Hutchison CJ. Internal lamin structures within G1 nuclei of human dermal fibroblasts. J Cell Sci. 1993 Feb;104 ( Pt 2):297-306. PubMed PMID: 8505362. Jenkins H, Hölman T, Lyon C, Lane B, Stick R, Hutchison C. Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix. J Cell Sci. 1993 Sep;106 ( Pt 1):275-85. PubMed PMID: 7903671. Hozák P, Sasseville AM, Raymond Y, Cook PR. Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells. J Cell Sci. 1995 Feb;108 ( Pt 2):635-44. PubMed PMID: 7769007. Machiels BM, Broers JL, Raymond Y, de Ley L, Kuijpers HJ, Caberg NE, et al. Abnormal A-type lamin organization in a human lung carcinoma cell line. Eur J Cell Biol. 1995 Aug;67(4):328-35. PubMed PMID: 8521872. Machiels BM, Zorenc AH, Endert JM, Kuijpers HJ, van Eys GJ, Ramaekers FC, et al. An alternative splicing product of the lamin A/C gene lacks exon 10. J Biol Chem. 1996 Apr 19;271(16):9249-53. PubMed PMID: 8621584. Machiels BM, Ramaekers FC, Kuijpers HJ, Groenewoud JS, Oosterhuis JW, Looijenga LH. Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults. J Pathol. 1997 Jun;182(2):197-204. PubMed PMID: 9274531. Jansen MP, Machiels BM, Hopman AH, Broers JL, Bot FJ, Arends JW, et al. Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease. Histopathology. 1997 Oct;31(4):304-12. PubMed PMID: 9363444. Broers JL, Machiels BM, Kuijpers HJ, Smedts F, van den Kieboom R, Raymond Y, et al. A- and B-type lamins are differentially expressed in normal human tissues. Histochem Cell Biol. 1997 Jun;107(6):505-17. PubMed PMID: 9243284.
Storage	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
Format	Buffer System: PBS with 0.09% Sodium Azide as preservative. State: Liquid purified IgG fraction. Purified
Specificity	Specificity: Reacts with an epitope located in the C-terminal part of Lamin B2. Species: Human, Mouse, Hamster, Swine, Xenopus, Zebrafish.
Gene ID	84823
FocusOn and Reviews	FocusOns: Antibodies to Lamins - FocusOn 028 Organelle Markers: Antibodies to the Nucleus - FocusOn 098
Targets	Lamin-B2 (LMNB2) antibody

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