LC3

Product Description for LC3

Rabbit anti Human, Mouse, Rat LC3.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for LC3

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	ICC/IF, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q9H492 Immunogen: The antiserum was produced against a synthesized peptide Corresponding to the amino terminal sequence from Human LC3A protein. AA Sequence: `MPSDRPFKQRRSFA` Genename: 84557
Antibody type	Ab
Application	Western blot: 1/500~1/1000. Immunofluorescence: 1/100~1/200.
Background	Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.
Concentration	1.0 mg/ml
General Readings	Hammarback JA, et al. Neuron 7(1):129-39, 1991. He H, et al. J. Biol Chem 278(31):29278-87, 2003.
Storage	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Purification: Immunoaffinity Chromatography using epitope-specific immunogen Buffer System: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Liquid purified Ig fraction Aff - Purified
Species Reactivity	Species reactivity (tested): Human, Mouse and Rat
Specificity	Specificity: This antibody detects endogenous levels of total LC3A/MAP1LC3A protein.
Gene ID	84557
FocusOn and Reviews	FocusOns: Autophagy - Recycling as a Survival Factor - FocusOn128
Targets	LC3 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for LC3 (8 products)

Catalog No.	Species	Pres.	Purity	Source
AR09594PU-L	LC3 (1-120, His-tag)
	Human	Purified	> 95 %	E. coli	0.5 mg / €1,000.00
					Acris Antibodies GmbH
AR09594PU-N	LC3 (1-120, His-tag)
	Human	Purified	> 95 %	E. coli	0.1 mg / €370.00
					Acris Antibodies GmbH
NBP1-45308-0.1	LC3
	Human	Purified			0.1 mg / €390.00
					Novus Biologicals Inc.
NBP1-45308-0.5	LC3
	Human	Purified			0.5 mg / €970.00
					Novus Biologicals Inc.
H00084557-P01-10	LC3
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00084557-P01-25	LC3
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00084557-Q01-10	LC3
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00084557-Q01-25	LC3
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for LC3 (2 products)

Catalog No.	Species
NBL1-12843	LC3 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00084557-T01	MAP1LC3A 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for LC3 (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH