MMP-1

Product Description for MMP-1

Mouse anti Human MMP-1 SB12e.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for MMP-1

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	CLG, Fibroblast collagenase, Interstitial collagenase, MMP1, Matrix metalloproteinase-1, Review 001
Presentation	Purified
Reactivity	Hu
Applications	C, E, P, WB
Clonality	Monoclonal
Clone	SB12e
Host	Mouse
Isotype	IgG2b
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P03956 Immunogen: Recombinant MMP-1. Genename: 4312
Antibody type	Ab
Add. information	Predicted Molecular Weight: 54 kDa.
Application	ELISA: 1/3,000-1/8,000. Western Blot. Immunohistochemistry (Frozen/Paraffin): ≤ 2 µg/ml.
Background	The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate the breakdown of the interstitial collagens, types I, II, and III
Concentration	0.5 mg/ml
General Readings	Welgus, H.G., C.J. Fliszar, J.L. Seltzer, T.M. Schmid, and J.J. Jeffery. 1990. J. Biol. Chem. 265:13521. Fosang, A.J., K. Last, V. Knauper, P.J. Neame, G. Murphy, T.E. Hardingham, H. Tschesche, and J.A. Hamilton. 1993. Biochem. J. 295:273. Perides, G., R.A. Asher, M.W. Lark, W.S. Lane, R.A. Robinson, and A. Bignami. 1995. Biochem. J. 312:377. Nguyen, Q., G. Murphy, C.E. Hughes, J.S. Mort, and P.J. Roughley. 1993. Biochem. J. 295:595. Imai, K., M. Kusakabe, T. Sakakura, I. Nakanishi, and Y. Okada. 1994. FEBS Letters 352:216. Sires, U.I., G. Murphy, V.M. Baragi, C.J. Fliszar, H.G. Welhus, and R.M. Senior. 1994. Biochem. Biophys. Res. Comm. 204:613. Desrochers, P.E., J.J. Jeffery, and S.J. Weiss. 1991. J. Clin. Invest. 87:2258. Mast. A.E., J.J. Enghild, H. Nagase, K. Suzuki, S.V. Pizzo, and G. Salvesen. 1991. J. Biol. Chem. 266:15810. Sires, U.I., G.L. Griffin, T.J. Broekelmann, R.P. Mecham, G. Murphy, A.E. Ching, H.G. Welhus, and R.M. Senior. 1993. J. Biol. Chem. 268:2069. Preece, G., G. Murphy, and A. Ager. 1996. J. Biol. Chem. 271:11634. Ito, A., A. Mukaiyama, Y. Itoh, H. Magase, I.B. Thogersen, J.J. Enghild, Y. Sasaguri, and Y. Mori. 1996. J. Biol. Chem. 271:14657. Fowlkes, J.L., J.J. Enghild, K. Suzuki, and H. Nagase. 1994. J. Biol. Chem. 269:25742. Chandler, S., R. Coates, A. Gearing, J. Lury, G. Wells, and E. Bone. 1995. Neurosci. Lett. 201:223. Thrailkill, K.M., L.D. Quarles, H. Nagase, K. Suzuki, D.M. Serra, and J.L. Fowlkes. 1995. Endocrinology 136:3527. Crabbe, T., J.P. O’Connell, B.J. Smith, and A.J. Docherty. 1994. Biochemistry 33:11419.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Liquid purified Ig fraction Purified
Specificity	Specificity: This antibody is specific for Human MMP-1. Does not cross react with Human MMP-2, MMP-3 or MMP-9. Other species not tested.
FocusOn and Reviews	Reviews: Antibodies to Matrix Metalloproteinases - Product Review 01
Targets	MMP-1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-1 (6 products)

Catalog No.	Species	Pres.	Purity	Source
AR09435PU-N	MMP-1
	Human		> 90 %	E. coli	10 µg / €230.00
					Acris Antibodies GmbH
AR09435PU-S	MMP-1
	Human		> 90 %	E. coli	2 µg / €130.00
					Acris Antibodies GmbH
H00004312-P01-10	MMP-1
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00004312-P01-25	MMP-1
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00004312-Q01-10	MMP-1
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00004312-Q01-25	MMP-1
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for MMP-1 (2 products)

Catalog No.	Species
H00004312-T01	MMP1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-13149	MMP1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for MMP-1 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH