AP00545PU-N MMP-1 antibody

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0.5 mg / €450.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human MMP-1

Product Description for MMP-1

Rabbit anti Human MMP-1.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for MMP-1

Product Category Antibodies
Quantity 0.5 mg
Synonyms CLG, Fibroblast collagenase, Interstitial collagenase, MMP1, Matrix metalloproteinase-1, Review 001
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P03956
Immunogen:
A synthetic peptide from the middle region of the Human MMP-1.
Genename:
4312
Remarks:
Mol. Wt of Antigen: 52 kDa (unglycosylated) and 57 kDa (glycosylated) species of pro form of MMP-1 plus 42 kDa (unglycosylated) and 47 kDa (glycosylated) active form of MMP-1.
Antibody type Ab
Application Western Blotting: 5-10 µg/ml for 2 hrs at RT.
Immunohistology (Formalin/Paraffin): Use 1/25-1/50 for 20 min at RT.
Requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended Positive Control: Conditioned, serum-free medium from (TPA-treated) HFL-1 cells. Placenta, Bladder, Breast, Ovarian Carcinoma.
Background Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) are implicated in the invasive potential of malignant tumors. Interstitial collagenase (MMP-1), 72 kDa gelatinase A/type IV collagenase (MMP-2), and 92 kDa gelatinase B/type IV collagenase (MMP-9) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.
Concentration 1.0 mg/ml
General Readings
  • Birkedal-Hansen B, et. al. Biochemistry, 1988;27:6751-6758.
  • Windsor LJ, et. al. Biochemistry, 1991;30:641-647.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% Sodium Azide.
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody detects MMP-1.
Cellular Localization: Cytoplasmic.
Species:
Human. Does not react with Cow.
Gene ID 4312
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-1 (6 products)

Catalog No. Species Pres. Purity   Source  
AR09435PU-N

MMP-1

MMP-1 Human > 90 % E. coli
10 µg / €230.00
  Acris Antibodies GmbH
AR09435PU-S

MMP-1

MMP-1 Human > 90 % E. coli
2 µg / €130.00
  Acris Antibodies GmbH
H00004312-P01-10

MMP-1

MMP-1 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00004312-P01-25

MMP-1

MMP-1 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
H00004312-Q01-10

MMP-1

MMP-1 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00004312-Q01-25

MMP-1

MMP-1 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for MMP-1 (2 products)

Catalog No. Species Pres. Purity   Source  
H00004312-T01

MMP1 293T Cell Transient Overexpression Lysate(Denatured)

MMP1 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-13149

MMP1 Lysate

Western Blot: MMP1 Lysate [NBL1-13149] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for MMP1
0.1 mg / €370.00
  Novus Biologicals Inc.