AF8810 MMP-15 antibody

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0.1 mg / €300.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Mouse MMP-15 162-22G5

Product Description for MMP-15

Mouse anti Mouse MMP-15 162-22G5.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for MMP-15

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms MMP15, MT-MMP 2, MT2-MMP, MT2MMP, MTMMP2, Matrix metalloproteinase-15, Membrane-type matrix metalloproteinase 2, Membrane-type-2 matrix metalloproteinase, SMCP-2
Presentation Purified
Reactivity Ms
Applications C, P, WB
Clonality Monoclonal
Clone 162-22G5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O54732
Immunogen:
Oligopeptide of DTDNFQLPEDDLRG (residue 281-294) on Mouse membrane-type 2 matrix metalloproteinase (MT2-MMP).
Genename:
17388
Antibody type Ab
Isotype control AM03095PU-N
Application Western Blotting: 5-10 µg/ml. 
Immunohistochemistry on Paraffin Sections: 2-20 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Ueno H, Nakamura H, Inoue M, Imai K, Noguchi M, Sato H, et al. Expression and tissue localization of membrane-types 1, 2, and 3 matrix metalloproteinases in human invasive breast carcinomas. Cancer Res. 1997 May 15;57(10):2055-60. PubMed PMID: 9158005.
  2. Nakamura H, Ueno H, Yamashita K, Shimada T, Yamamoto E, Noguchi M, et al. Enhanced production and activation of progelatinase A mediated by membrane-type 1 matrix metalloproteinase in human papillary thyroid carcinomas. Cancer Res. 1999 Jan 15;59(2):467-73. PubMed PMID: 9927064.
General Readings
  1. Bassi DE, Lopez De Cicco R, Cenna J, Litwin S, Cukierman E, Klein-Szanto AJ. PACE4 expression in mouse basal keratinocytes results in basement membrane disruption and acceleration of tumor progression. Cancer Res. 2005 Aug 15;65(16):7310-9. PubMed PMID: 16103082.
  2. Mahloogi H, Bassi DE, Klein-Szanto AJ. Malignant conversion of non-tumorigenic murine skin keratinocytes overexpressing PACE4. Carcinogenesis. 2002 Apr;23(4):565-72. PubMed PMID: 11960907.
  3. Nomura H, Sato H, Seiki M, Mai M, Okada Y. Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. Cancer Res. 1995 Aug 1;55(15):3263-6. PubMed PMID: 7614460.
  4. Tanaka M, Sato H, Takino T, Iwata K, Inoue M, Seiki M. Isolation of a mouse MT2-MMP gene from a lung cDNA library and identification of its product. FEBS Lett. 1997 Feb 3;402(2-3):219-22. PubMed PMID: 9037199.
Storage Upon receipt, store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Stabilizers:
0.5% protease free BSA
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
The monoclonal 162-22G5 antibody specifically reacts with Mouse MT2-MMP and cross reacts with Human MT2-MMP.
Gene ID 17388
FocusOn and Reviews
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