MMP-2

Product Description for MMP-2

Mouse anti Human, Mouse, Rat MMP-2 MMP2/2C1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for MMP-2

Product Category	Antibodies
Quantity	25 µl
Synonyms	72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, Gelatinase A, MMP2, Matrix metalloproteinase-2, Review 001, TBE-1
Presentation	Purified
Reactivity	Hu, Ms, Rt
Applications	C, E, ICC/IF, IP, P, WB
Clonality	Monoclonal
Clone	MMP2/2C1
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P08253 Immunogen: Activated recombinant human MMP-2. Genename: 4313
Antibody type	Ab
Add. information	Research Areas: Hypoxia, Cancer
Application	ELISA, Immunohistochemistry-Frozen, Western Blot, NB 200-113 may be used for Western blotting, where a band is seen at 70 and 62 kDa representing the MMP-2 band-pair. In addition, this antibody may be used for immunohistochemistry (frozen tissue), ELISA, and immunoprecipitation. ELISA and immunoprecipitation have only been tested on human and recombinant protein. This antibody is not recommended for immunohistocheistry on praffin sections. See catalog no. NB 200-114 for IHC in paraffin. Suggested working dilutions: * Western Blot 2-5 ug/ml ELISA <0.1 ug/ml Immunohistochemistry 5 ug/ml The investigator should determine the optimal working dilution for a specific application. *Works on frozen tissue. For paraffin, see product NB 200-214. , ELISA 1 ug/ml, Western Blot 2-5 ug/ml, Immunohistochemistry-Frozen 5 ug/ml
Background	Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, a protein involved in the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus, detection of the enzyme is a possible early indicator of tumor activity.
Concentration	1 mg/ml
General Readings	Zuo J, Ferguson TA, Hernandez YJ, Stetler-Stevenson WG, Muir D. Neuronal matrix metalloproteinase-2 degrades and inactivates a neurite-inhibiting chondroitin sulfate proteoglycan. J Neurosci. 1998 Jul 15;18(14):5203-11. PubMed PMID: 9651203. Ferguson TA, Muir D. MMP-2 and MMP-9 increase the neurite-promoting potential of schwann cell basal laminae and are upregulated in degenerated nerve. Mol Cell Neurosci. 2000 Aug;16(2):157-67. PubMed PMID: 10924258. Muir D. Differences in proliferation and invasion by normal, transformed and NF1 Schwann cell cultures are influenced by matrix metalloproteinase expression. Clin Exp Metastasis. 1995 Jul;13(4):303-14. PubMed PMID: 7606893.
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: protein G purified Buffer System: 0.05% Sodium Azide Purity: protein G purified State: Purified
Specificity	Specificity: This is specific for pro and active MMP-2. Species: Human, Mouse, Rat
Gene ID	4313
FocusOn and Reviews	Reviews: Antibodies to Matrix Metalloproteinases - Product Review 01
Targets	MMP-2 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-2 (4 products)

Catalog No.	Species	Pres.	Source
H00004313-P01-10	MMP-2
	Human	Purified		10 µg / €350.00
				Abnova Taiwan Corp.
H00004313-P01-25	MMP-2
	Human	Purified		25 µg / €500.00
				Abnova Taiwan Corp.
H00004313-Q01-10	MMP-2
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00004313-Q01-25	MMP-2
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.

Recommended Secondary Antibodies for MMP-2 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH