MMP-3

Product Description for MMP-3

Mouse anti Human MMP-3 SB14d.
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Paraffin Sections.

Properties for MMP-3

Product Category	Antibodies
Quantity	1 ml
Synonyms	MMP3, Matrix metalloproteinase-3, Review 001, SL-1, STMY1, Stromelysin-1, Transin-1
Presentation	HRP
Reactivity	Hu
Applications	C, E, P
Clonality	Monoclonal
Clone	SB14d
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P08254 Immunogen: Recombinant MMP-3. Genename: 4314
Antibody type	Ab
Application	ELISA: 1/1,000-1/4,000. Immunohistochemistry (Frozen/Paraffin): 1/1,000-1/2,000.
Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer.
General Readings	Nicholson, R., G. Murphy, and R. Breathnach. 1989. Biochemistry 28:5195. Murphy, G., M.I. Cockett, R.V. Ward, and A.J. Docherty. 1991. Biochem. J. 277:277. Okada, Y., H. Konomi, T. Yada, K. Kimata, and H. Nagase. 1989. FEBS Lett. 244:473. Chin, J.R., G. Murphy, and Z. Werb. 1985. J. Biol. Chem. 260:12367. Windsor, L.J., H. Grenett, B. Birkedal-Hansen, M.K. Bodden, J.A. Engler, and H. Birkedal-Hansen. 1993. J. Biol. Chem. 268:17341. Bini, A., Y. Itoh, B.J. Kudryk, and H. Nagase. 1996. Biochemistry 35:13056. Lijnen, H.R., F. Ugwu, A. Bini, and D. Collen. 1998. Biochemistry 37:4699. Sires, U.I., G.L. Griffin, T.J. Broekelmann, R.P. Mecham, G. Murphy, A.E. Ching, H.G. Welhus, and R.M. Senior. 1993. J. Biol. Chem. 268:2069. Chandler, S., R. Coates, A. Gearing, J. Lury, G. Wells, and E. Bone. 1995. Neurosci. Lett. 201:223. Ito, A., A. Mukaiyama, Y. Itoh, H. Magase, I.B. Thogersen, J.J. Enghild, Y. Sasaguri, and Y. Mori. 1996. J. Biol. Chem. 271:14657. Mitchell, T.I., J.J. Jeffrey, R.D. Palmiter, and C.E. Brinckerhoff. 1993. Biochim. Biophys. Acta. 1156:245. Murphy, G., M.I. Cockett, P.E. Stephens, B.J. Smith, and A.J. Docherty. 1987. Biochem. J. 248:265. Miyazaki, K., F. Umenishi, K. Funahashi, N. Koshikawa, U. Yasumitsu, and M. Umeda. 1992. Biochem. Biophys. Res. Commun. 185:852. Imai, K., Y. Yokohama, I. Nakanishi, E. Ohuchi, Y. Fujii, N. Nakai, and Y. Okada. 1995. J. Biol. Chem. 270:6691. Knauper, V., S.M. Wilhelm, P.K. Seperack. Y.A. DeClerck, K.E. Langley, A. Osthues, and H. Tschesche. 1993. Biochem. J. 295:581. Shapiro, S.D., C.J. Fliszar, T.J. Broekelmann, R.P. Mecham, R.M. Senior, and H.G. Welgus. 1995. J. Biol. Chem. 270:6351. Knauper, V., C. Lopez-Otin, B. Smith, G. Knight, and G. Murphy. 1996. J. Biol. Chem. 271:1544.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Horseradish Peroxidase HRP Buffer System: 50% Glycerol/50% PBS, pH 7.4 without preservatives. State: Liquid purified Ig fraction.
Specificity	Specificity: This antibody is specific for Human MMP-3. Does not cross react to Human MMP-1, MMP-2 or MMP-9. Species: Human.
Gene ID	4314
FocusOn and Reviews	Reviews: Antibodies to Matrix Metalloproteinases - Product Review 01
Targets	MMP-3 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-3 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09439PU-N	MMP-3
	Human		> 98 %	E. coli	10 µg / €230.00
					Acris Antibodies GmbH
AR09439PU-S	MMP-3
	Human		> 98 %	E. coli	2 µg / €130.00
					Acris Antibodies GmbH
PA1265X	MMP-3
	Human	Purified	> 98 %	E. coli	Regular Price: 20 µg / €170.00 Special Price: 20 µg / €119.00
					Acris Antibodies GmbH
H00004314-P01-10	MMP-3
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00004314-P01-25	MMP-3
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00004314-Q01-10	MMP-3
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00004314-Q01-25	MMP-3
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for MMP-3 (2 products)

Catalog No.	Species
H00004314-T01	MMP3 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-13159	MMP3 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for MMP-3 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH