MMP-3

Product Description for MMP-3

Rabbit anti Human, Mouse, Rat MMP-3 EP1186Y.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for MMP-3

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	MMP3, Matrix metalloproteinase-3, Review 001, SL-1, STMY1, Stromelysin-1, Transin-1
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	ICC/IF, P, WB
Clonality	Monoclonal
Clone	EP1186Y
Host	Rabbit
Isotype	IgG
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P08254 Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human MMP3. Genename: 4314
Antibody type	Ab
Add. information	Research Areas: Cancer
Application	Immunocytochemistry, immunohistochemistry, Western Blot, WB: 1:1000 IHC: 1:100 , immunohistochemistry , Western Blot , Immunocytochemistry
Background	Effective inhibitors of matrix metalloproteinases (MMPs), a family of connective tissue-degrading enzymes, could be useful for the treatment of diseases such as cancer, multiple sclerosis, and arthritis (1). Matrix metalloproteinase-3 (MMP-3) is a protein involved in tumor invasion and metastasis. MMP3 (stromelysin) is a secreted metalloprotease with a wide range of substrate specificities. MMP3 is synthesized in a preproenzyme form with a calculated size of 54 kDa and a 17-amino acid long signal peptide. Data indicate that the expression and the possible involvement of secreted metalloproteases in tumorigenesis result from a specific interaction between the transforming factor and the target cell, which may vary in different species (2). In neural studies, matrix metalloproteinase-3 (MMP-3) was newly induced and activated in stressed dopamine cells, and the active form of MMP-3 (actMMP-3) was released into the medium. The released actMMP-3, as well as catalytically active recombinant MMP-3 (cMMP-3) led to microglial activation and superoxide generation in microglia and enhanced DA cell death. (3).
General Readings	Wilhelm SM, et al. Proc Natl Acad Sci USA 84(19):6725-9, Pavlovsky AG, et al. Protein Sci, 8(7):1455-62, 1999. Kim YS, et al. FASEB J 21(1):179-87, 2007.
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: Tissue culture supernatant Buffer System: 0.01% Sodium Azide Purity: Tissue culture supernatant State: Aff - Purified
Specificity	Specificity: A synthetic peptide corresponding to residues near the C-terminus of human MMP-3 was used as an immunogen. Species: Human, Mouse, Rat
Gene ID	4314
FocusOn and Reviews	Reviews: Antibodies to Matrix Metalloproteinases - Product Review 01
Targets	MMP-3 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for MMP-3 (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09439PU-N	MMP-3
	Human		> 98 %	E. coli	10 µg / €230.00
					Acris Antibodies GmbH
AR09439PU-S	MMP-3
	Human		> 98 %	E. coli	2 µg / €130.00
					Acris Antibodies GmbH
PA1265X	MMP-3
	Human	Purified	> 98 %	E. coli	Regular Price: 20 µg / €170.00 Special Price: 20 µg / €119.00
					Acris Antibodies GmbH
H00004314-P01-10	MMP-3
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00004314-P01-25	MMP-3
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00004314-Q01-10	MMP-3
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00004314-Q01-25	MMP-3
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for MMP-3 (2 products)

Catalog No.	Species
H00004314-T01	MMP3 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.
NBL1-13159	MMP3 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for MMP-3 (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH