MRP8/14 (S100A8/A9) - BM4025X | acris-antibodies.com

Product Description for MRP8/14 (S100A8/A9)

Mouse anti Human MRP8/14 (S100A8/A9) 27E10.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Paraffin Sections.

Properties for MRP8/14 (S100A8/A9)

Product Category	Antibodies
Quantity	1 mg
Synonyms	CAGA, CAGB, CFAG, Calgranulin A/B, Calprotectin, FocusOn041, L1 Protein, MRP-14, MRP-8, P14, P8
Presentation	Purified
Reactivity	Hu
Applications	C, E, P
Clonality	Monoclonal
Clone	27E10
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Cultured Human monocytes. Remarks: The antigen is MRP8/14 (Calprotectin), the epitope involves parts of both subunits MRP8 and MRP14.
Antibody type	Ab
Application	ELISA. Immunohistochemistry (Including Paraffin Sections after Predigestion with Trypsin or Proteinase K): 1 µg/ml Fixation: Acetone, Formalin/Paraffin.
Background	The antigen is produced by the heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
Concentration	1.8 mg/ml (by Absorbance at 280 nm)
Protocols	Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS 6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS 13. Counterstain with Mayer’s
Product Citations	1. Altwegg LA, Neidhart M, Hersberger M, Müller S, Eberli FR, Corti R, et al. Myeloid-related protein 8/14 complex is released by monocytes and granulocytes at the site of coronary occlusion: a novel, early, and sensitive marker of acute coronary syndromes. Eur Heart J. 2007 Apr;28(8):941-8. Epub 2007 Mar 26. PubMed PMID: 17387139. FITC conjugated antibody is cited in: 2. Ghavami S, Rashedi I, Dattilo BM, Eshraghi M, Chazin WJ, Hashemi M, et al. S100A8/A9 at low concentration promotes tumor cell growth via RAGE ligation and MAP kinase-dependent pathway. J Leukoc Biol. 2008 Jun;83(6):1484-92. doi: 10.1189/jlb.0607397. Epub 2008 Mar 13. PubMed PMID: 18339893.
General Readings	Zwadlo G, Schlegel R, Sorg C. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol. 1986 Jul 15;137(2):512-8. PubMed PMID: 3722815. Steinhoff, G. et al.: Patterns of macrophage immigration and differentiaton in human liver grafts. Transplant. Proc. 21(1), 398-400 (1989). Johne B, Fagerhol MK, Lyberg T, Prydz H, Brandtzaeg P, Naess-Andresen CF, et al. Functional and clinical aspects of the myelomonocyte protein calprotectin. Mol Pathol. 1997 Jun;50(3):113-23. PubMed PMID: 9292145.
Storage	Stock solution or aliquots thereof can be kept frozen at -20°C. Avoid repeated freezing and thawing. Do Not Freeze working dilutions. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer System: PBS, pH 7.2 Preservatives: 0.05% Sodium Azide State: Liquid purifed Ig fraction (> 95% pure by SDS- PAGE) Purified
Species Reactivity	Species reactivity (tested): Human. The antibody also stains a subpopulation of macrophages of Rhesus Monkey and Bovine tissues. It does not react with Swine tissues.
Specificity	Specificity: This antibody is ideally suited for the detection of early inflammatory macrophages and thus for the classification of acute stage inflammation in tissue sections and in smears, the characterization of tumorous tissues and the in vitro monitoring of peripheral blood cell cultures. Clone 27E10 is unique in that it recognizes an epitope on the MRP8/14 heterocomplex that is not exposed on the individual subunits MRP8 or MRP14. The antibody reacts with Human subpopulations of macrophages, monocytes and granulocytes; peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Antigen Distribution: Isolated Cells: A subpopulation of monocytes and neutrophilic granulocytes are positive. Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80% of monocytes in early cultures (24-48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue Sections: The antigen is found in macrophages in the red pulp of the spleen and in the liver; strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen; absent on normal resident mononuclear phagocytes in healthy tissues (skin, gut, thymus).
FocusOn and Reviews	FocusOns: Antibodies to S100 Family - FocusOn 041
Targets	MRP8 / MRP14 antibody

Accessory Products

Recommended Secondary Antibodies for MRP8/14 (S100A8/A9) (8 products)

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R1253HRP	Mouse IgG (H+L chain) antibody
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R1253TR	Mouse IgG (H+L chain) antibody
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R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
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