MRP8 / MRP14 - SM2011PS | acris-antibodies.com

Product Description for MRP8 / MRP14

Mouse anti Bovine, Canine, Equine, Feline, Guinea Pig, Human, Monkey, Porcine, Rabbit, Rat MRP8 / MRP14 MAC387.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for MRP8 / MRP14

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	MRP8/14, S100A8, S100A8/A9, S100A9
Presentation	Aff - Purified
Reactivity	Bov, Can, Eq, Fe, GP, Hu, Mky, Por, Rb, Rt
Applications	C, F, P
Clonality	Monoclonal
Clone	MAC387
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Human Monocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Antibody type	Ab
Application	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 1x10⁶ cells in 100 µl (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections: 1/100-1/200. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200. This antibody requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment prior to staining. Recommended Positive Control: Human Spleen Tissue.
Background	Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becomming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognised as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
Concentration	1.0 mg/ml
General Readings	1. Brandtzaeg, P., et al (1988). MAC387 antibody and detection of formalin resistant myelomonocytic L1 antigen J. Clin. Path. 41: 963-970. 2. Brandtzaeg, P. et al (1992). The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative biological function. Histopathol. 21: 191-196. 3. Flavell, D.J., Jones, D.B., Wright, D.H. (1987). Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35: 1217-1226. 4. Gutierrez, M. et al. (1999). The detection of CD2+, CD4+, CD8+ and WC1+, Tlymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334. 5. Ramsay, A. D. et al. (1991). Phenotypic analysis of malignant lymphoma in simian immunodeficiency virus infection using anti-human antibodies. Journal of Pathology. 164: 321 - 328. 6. Christgau, M. et al. (1998). Characterization of Immunocompetent cells in the diseased canine periodontium. Journal of Histochemistry & Cytochemistry. 46 (12): 1443-1454. 7. Perez, J. et al. (1999). Immunohistochemical study of the inflammatory infiltrate assocated with equine squamous cell carcinoma. J. Comp. Path. 121: 385-397. 8. Obert, L. et al. (2002). Early pathogenesis of transmucosal Feline Immunodeficiency Virus infection. J. Virol. 76 (12): 6311-6322. 9. Malik, N. et al. (1998). Apoptosis and Cell proliferation after porcine coronary angioplasty. Circulation. 98: 1657-1665. 10. Bagavant, H. et al. (2002). Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis)). Am. J. Pathol. 160: 141-149.
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer Solution: PBS Preservatives: 0.09% Sodium Azide State: Liquid purified IgG fraction Aff - Purified
Species Reactivity	Species reactivity (tested): Human, Horse, Porcine, Canine, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Feline, Cynomologus monkey, Rhesus monkey and Goat.
Specificity	Specificity: This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain expressed by Granulocytes, Monocytes and by tissue Macrophages. Variable results have been reported for staining brain macrophages and microglia.
FocusOn and Reviews	FocusOns: Antibodies to Human Macrophages - FocusOn 027
Targets	MRP8 / MRP14 antibody

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