Neurofilament M (160 kD) - SM3068P | acris-antibodies.com

Product Description for Neurofilament M (160 kD)

Mouse anti All Species Neurofilament M (160 kD) NF-09.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Neurofilament M (160 kD)

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	(Neuronal Marker), FocusOn130, NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
Presentation	Purified
Reactivity	All Species
Applications	ICC/IF, P, WB
Clonality	Monoclonal
Clone	NF-09
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P08552 Immunogen: Pellet of Porcine brain cold stable proteins after depolymerization of microtubules
Antibody type	Ab
Application	Western blot. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. Positive Tissue: Neuro2A murine cell line. Note: Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min.
Background	Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.
Concentration	1.0 mg/ml
General Readings	1. Draberova E, Sulimenko V, Kukharskyy V, Draber P.: Monoclonal antibody NF-09 specific for neurofilament protein NF-M. Folia Biol (Praha). 1999;45(4):163-5. 2. Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T.: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95. 3. Rao MV, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, Nixon RA: The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate. J Cell Biol. 2003 Dec 8;163(5):1021-31. 4. Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX: Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease. FASEB J. 2008 Jan;22(1):138-45.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein A Buffer System: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative. State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Purified
Specificity	Specificity: The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable. Species: Mammalian.
FocusOn and Reviews	FocusOns: Antibodies for Alzheimer’s Disease - FocusOn 130
Targets	Neurofilament M (160 kD) antibody

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