AP22066PU-N NUP35 (280-330) antibody

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0.1 mg / €320.00
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Rabbit anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Opossum NUP35

AP22066PU-N

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  • AP22066PU-N

Product Description for NUP35

Rabbit anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Opossum NUP35.
Properties: (280-330)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for NUP35

Product Category Antibodies
Quantity 0.1 mg
Synonyms 35 kDa nucleoporin, FocusOn098, MP44, Mitotic phosphoprotein 44, NUP53, Nuclear pore complex protein Nup53, Nucleoporin NUP53, Nucleoporin Nup35
Presentation Purified
Reactivity Bov, Can, Chk, Hu, Mky, Ms, Op
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8NFH5
Immunogen:
Amino acids 280-330 of human NUP35
Genename:
129401
Remarks:
The amino acid sequence used as immunogen is 100% homologous in human, rat, mouse, cow, chimpanzee, monkey and dog, 93% homologous in opossum and chicken and 81% homologous in zebrafish.
Property 280-330
Antibody type Ab
Application Immunohistochemistry on paraffin sections: 5 µg/ml
Western blot: 1-3 µg/ml.
Recommended positive control: Liver lysate.
Background Nucleoporin Nup35, or Mitotic phosphoprotein 44, is a nuclear protein known to be present on the nuclear pore complex and is tightly associated with the nuclear membrane and lamina. This RNA-associated protein belongs to the Nup53 family and has a MPPN (mitotic phosphoprotein N' end) domain. It has a small number of scattered phenylalanine-glycine repeats, but not the large domains seen in other nucleoporins. Though the exact function of NUP35 is yet to be revealed, it may function as a component of the nuclear pore complex (NPC) which are collectively referred to as nucleoporins (NUPs). It also may play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors, and may also play an important part in the association of MAD1, a transcriptional repressor with the NPC. NUP35 is widely expressed in most of the tissues.
Concentration 0.5 mg/ml
General Readings
  • Cronshaw, JM. et al. J. Cell Biol. 158:915-927 (2002).
  • Hawryluk-Gara, LA. et al. Mol. Biol. Cell. 16:2382-2394 (2005).
  • Dephoure, N. et al. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767 (2008).
Storage Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A chromatography
Buffer System:
PBS containing 0.05% BSA and 0.05% sodium azide
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (expected):
Chicken, Opossum, Zebrafish
Species reactivity (tested):
Chimpanzee, Cow, Dog, Human, New World monkey, Mouse
Specificity
Specificity:
This antibody detects NUP35.
Gene ID 129401
FocusOn and Reviews
FocusOns:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for NUP35 (3 products)

Catalog No. Species Pres. Purity   Source  
H00129401-P01

NUP35

NUP35 Human Purified
2 µg / €250.00
  Abnova Taiwan Corp.
H00129401-Q01-10

NUP35

NUP35 Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00129401-Q01-25

NUP35

NUP35 Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for NUP35 (1 products)

Catalog No. Species Pres. Purity   Source  
H00129401-T01

NUP35 293T Cell Transient Overexpression Lysate(Denatured)

NUP35 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.