pan Cytokeratin - AM10028PU-N | acris-antibodies.com

Product Description for pan Cytokeratin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat pan Cytokeratin AE1/AE3.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for pan Cytokeratin

Product Category	Antibodies
Quantity	0.2 mg
Synonyms	Cytokeratin pan-reactive, pan Keratin
Presentation	Purified
Reactivity	Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt
Applications	C, ICC/IF, P
Clonality	Monoclonal
Clone	AE1/AE3
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52.5). + CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) CK8 (52.5).
Antibody type	Ab
Application	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentrated antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. For FFPE tissue sections antigen retriever like trypsin is required. Recommended Positive Control: Human skin, lung ca.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).
Concentration	0.2 mg/ml
General Readings	Moll, R et al Cell, 31: 11-24, (1982). Sun, TT et. al. Am. N Y Acad. Sci. 455: 307-329 (1985). Tseng, SC et.al. Cell 30: 361-372 (1982). Kuraguchi, M P et. al. PLoss Genet2:e146 (2006). Battifora, H et. al. 54: 843-848 (1984).
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Format	Purification: Protein A Chromatography. Buffer System: PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative. State: Liquid purified Ig fraction. Purified
Species Reactivity	Species reactivity (tested): Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.
Specificity	Specificity: This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5). Cellular Localization: Cytoplasmic.
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	pan Cytokeratin antibody

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