pan Cytokeratin (4+5+6+8+10+13+18) - BM555F

Product Description for pan Cytokeratin

Mouse anti Amphibian, Bovine, Human, Rat pan Cytokeratin C-11.
Properties: (4+5+6+8+10+13+18)
Presentation: FITC
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for pan Cytokeratin

Product Category	Antibodies
Quantity	100 Tests
Synonyms	Cytokeratin pan-reactive, pan Keratin
Presentation	FITC
Reactivity	Amph, Bov, Hu, Rt
Applications	C, F, ICC/IF, P, WB
Clonality	Monoclonal
Clone	C-11
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: Epidermal carcinoma cell line A431
Property	4+5+6+8+10+13+18
Antibody type	Ab
Application	Western blot. Immunofluorescence. Flow Cytometry: Use 10 µl of neat antibody to label 1x 10⁶ cells in 100 µl. Immunohistochemistry on Frozen Sections Immunohistochemistry on Paraffin Sections (Require protein digestion pre-treatment e. g. trypsin or pronase).
Background	Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
General Readings	Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305. Bártková J, Bártek J, Lukás Z, Vojtĕsek B, Stasková Z, Bursová H, et al. Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology. Neoplasma. 1991;38(4):439-46. PubMed PMID: 1717857.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Fluorescein Isothiocyanate Isomer 1 FITC Purification: Ion Exchange Chromatography followed by Gel Filtration Buffer Solution: PBS Preservatives: 0.09% Sodium azide Stabilizers: 1% BSA State: Liquid purified Ig fraction
Species Reactivity	Species reactivity (tested): Human, Rat, Amphibia and Bovine
Specificity	Specificity: This antibody recognises Cytokeratins 4, 5, 6, 8, 10, 13 and 18. The antibody reacts specifically with a wide range of normal, reactive and neoplastic epithelial tissues.
FocusOn and Reviews	Reviews: Antibodies to Cytokeratin Proteins - Product Review 09
Targets	pan Cytokeratin antibody

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