Product Description for PUMA
Rabbit anti Human PUMA.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Properties for PUMA
| Product Category | Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | BBC3, Bcl-2-binding component 3, JFY-1, Review 004, p53 up-regulated modulator of apoptosis |
| Presentation | Aff - Purified |
| Reactivity | Hu |
| Applications | P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | N-term |
| Antibody type | Ab |
| Add. information | Research Areas: Cancer, DNA Repair |
| Application | Western Blot, Western blot at 2 to 4 ug/ml. K562 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected., Western Blot |
| Background | Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. |
| General Readings | Stankovic T et al. Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses. Blood 103:291-300 (2004). |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Format |
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| Specificity |
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| Gene ID | 27133 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for PUMA (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| GTX29644 |
PUMA |
||||||
 |
50 µg /
€250.00
|
||||||
| GeneTex Inc. | |||||||
| GTX29646 |
PUMA |
||||||
|
50 µg /
€230.00
|
||||||
| GeneTex Inc. | |||||||
Positive controls for PUMA (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07925 |
PUMA Lysate |
||||||
![Western Blot: PUMA Lysate [NBL1-07925] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for BBC3](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/PUMA Lysate-Western Blot-NBL1-07925-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for PUMA (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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