PUMA

Product Description for PUMA

Mouse anti Human, Mouse PUMA RB1353-RB1354.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for PUMA

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	BBC3, Bcl-2-binding component 3, JFY-1, Review 004, p53 up-regulated modulator of apoptosis
Presentation	Purified
Reactivity	Hu, Ms
Applications	E, F, IP, P, WB
Clonality	Monoclonal
Clone	RB1353-RB1354
Host	Mouse
Isotype	IgG
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: Q9BXH1 Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acid residues 134-154 of human Puma. Genename: 27113
Antibody type	Ab
Application	ELISA, flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, This antibody is useful for ELISA, Western blotting, Immunohistochemistry, Immunoprecipitation, flow cytometry / FACS analysis, flow cytometry / FACS analysis , ELISA , immunohistochemistry , immunoprecipitation , Western Blot
Background	Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl-2 family members, among others, play an important role.The Bcl-2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Members of this family include both pro- and anti-apoptotic proteins that hare homology sequences called Bcl-2 Homology domains (BH1-4) which mediate dimmer formation. The BH3 proteins, such as BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence homology within the amphipathic alpha-helical BH3 region, which is required for their apoptotic function. They may trigger release of death-inducing molecules such as Cytochrome C, Smac, and endonuclease G. Anti-apoptotic family members, including Bcl-2 and Bcl-XL, play inhibitory roles. Bcl-2 family proteins may form homodimers or heterodimers between pro- and anti-apoptotic members, the ratios of which determine the cell fate.
Concentration	0.25 mg/ml
Storage	Store at 4C. Do not freeze.
Format	Purification: IgG purified Buffer System: 0.02% Sodium Azide Purity: IgG purified State: Purified
Specificity	Specificity: Puma (RB1353-RB1354) Species: Human, Mouse
Gene ID	27113
FocusOn and Reviews	Reviews: Antibodies to bcl-2 Protein Family - Product Review 04
Targets	PUMA antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for PUMA (2 products)

Catalog No.	Species
GTX29644	PUMA
		50 µg / €250.00
		GeneTex Inc.
GTX29646	PUMA
		50 µg / €230.00
		GeneTex Inc.

Positive controls for PUMA (1 products)

Catalog No.	Species
NBL1-07925	PUMA Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for PUMA (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH