SP7151P PUMA alpha/beta (C-term) antibody

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0.1 mg / €310.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse PUMA

SP7151P

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  • SP7151P

Product Description for PUMA

Rabbit anti Human, Mouse PUMA.
Properties: alpha/beta (C-term)
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for PUMA

Product Category Antibodies
Quantity 0.1 mg
Synonyms BBC3, Bcl-2-binding component 3, JFY-1, Review 004, p53 up-regulated modulator of apoptosis
Presentation Purified
Reactivity Hu, Ms
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9BXH1
Immunogen:
A portion of amino acids 150-193 of Human PUMA alpha
AA Sequence:
PLPRGHRAPEMEPN
Genename:
27113
Remarks:
The amino acid sequence used as immunogen is 100% homologous in Human (PUMA alpha and beta) and 78% homologous in Mouse and Rat.
Property alpha/beta, C-term
Antibody type Ab
Application Western blot: 1/500-1/1,000.  
Immunohistochemistry on Paraffin Sections: See Karst et al, 2005.
Recommended Positive Control: Jurkat or NIH 3T3 cell lysate.
Background A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Concentration 0.5 mg/ml
General Readings
  • Nakano K., et al. Mol Cell. 7(3):683-94 (2001).
  • Yu J., et al. Mol Cell. Vol 7,673-682 (2001).
  • Han J., et al. Proc Natl Acad Sci U S A. 98(20):11318-23 (2001)
  • Karst AM, Dai DL, Martinka M, Li G. PUMA expression is significantly reduced in human cutaneous melanomas. Oncogene. 2005 Feb 3;24(6):1111-6. PubMed PMID: 15690057.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein G Chromatography
Buffer System:
PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.2% Gelatin
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for PUMA alpha/beta (C-term).
Species:
Human, Mouse.
Gene ID 27113
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for PUMA (2 products)

Catalog No. Species Pres. Purity   Source  
GTX29644

PUMA

PUMA
50 µg / €250.00
  GeneTex Inc.
GTX29646

PUMA

PUMA
50 µg / €230.00
  GeneTex Inc.

Positive controls for PUMA (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-07925

PUMA Lysate

Western Blot: PUMA Lysate [NBL1-07925] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for BBC3
0.1 mg / €370.00
  Novus Biologicals Inc.