Product Description for PUMA
Rabbit anti Human, Mouse PUMA.
Properties: alpha isoform (N-term)
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.
Properties: alpha isoform (N-term)
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.
Properties for PUMA
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | BBC3, Bcl-2-binding component 3, JFY-1, Review 004, p53 up-regulated modulator of apoptosis |
| Presentation | Purified |
| Reactivity | Hu, Ms |
| Applications | C, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | alpha isoform, N-term |
| Antibody type | Ab |
| Application | Western blot: 0.5-1.0 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Recommended Positive Control: 3T3 cells, K562 cells. |
| Background | A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) in human and mouse. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. |
| Concentration | 1 mg/ml |
| General Readings |
|
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
| Format |
|
| Specificity |
|
| Gene ID | 27113 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for PUMA (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| GTX29644 |
PUMA |
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50 µg /
€250.00
|
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| GeneTex Inc. | |||||||
| GTX29646 |
PUMA |
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![]() |
50 µg /
€230.00
|
||||||
| GeneTex Inc. | |||||||
Positive controls for PUMA (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07925 |
PUMA Lysate |
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0.1 mg /
€370.00
|
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for PUMA (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||


![Western Blot: PUMA Lysate [NBL1-07925] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for BBC3](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/PUMA Lysate-Western Blot-NBL1-07925-img0001.jpg)




