RFP-Tag (Ads. to Hu, Ms, Rt Serum Proteins) - AP09229PU-N | acris-antibodies.com

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Product Description for RFP-Tag

Rabbit anti RFP-Tag.
Properties: (Ads. to Hu, Ms, Rt Serum Proteins)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for RFP-Tag

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	DsRed Tag, Red fluorescent protein Tag
Presentation	Aff - Purified
Applications	E, P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: Q9U6Y8 Immunogen: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
Property	Ads. to Hu, Ms, Rt Serum Proteins
Antibody type	Ab
Application	Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, Fluorometry and Fluorescence Activated Cell Sorting (FACS). Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (Sandwich or Capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to Biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by Immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on Western blots. Recommended Dilutions: ELISA: 1/90,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/200-1/2,000.
Background	Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
Concentration	1.0 mg/ml (by UV absorbance at 280 nm)
General Readings	Gross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY. The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5. PubMed PMID: 11050230.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities Buffer System: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Preservatives: 0.01% (w/v) Sodium Azide Stabilizers: None State: Liquid (sterile filtered) purified Ig fraction Aff - Purified
Specificity	Specificity: Polyclonal anti-RFP is designed to detect RFP and its variants. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
Targets	RFP-Tag antibody

Accessory Products

Recommended Secondary Antibodies for RFP-Tag (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

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