Product Description for S100A1
Rabbit anti Bovine, Human, Mouse, Porcine, Rat S100A1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.
Properties for S100A1
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | FocusOn041, S-100 protein alpha chain, S-100 protein subunit alpha, S100 alpha, S100 calcium-binding protein A1, S100-A1, S100A |
| Presentation | Aff - Purified |
| Reactivity | Bov, Hu, Ms, Por, Rt |
| Applications | E, IP, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
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|---|---|
| Antibody type | Ab |
| Application | ELISA: Assay dependent. Immunoprecipitation (5 µg/ml). Immunohistochemistry: Formalin-fixed, Paraffin-Embedded Sections (1 µg/ml): Demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland. Western blotting (0.1 µg/ml): Detects a 10 kDa protein representing S100-alpha from Human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. |
| Background | The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells. |
| Product Citations |
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| General Readings |
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| Storage | Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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| Gene ID | 6271 |
| FocusOn and Reviews |
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| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for S100A1 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00006271-P01-02 |
S100A1 |
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Human | in vitro transl. |
2 µg /
€250.00
|
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| Abnova Taiwan Corp. | |||||||
| H00006271-Q01-10 |
S100A1 |
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Human | in vitro transl. |
10 µg /
€350.00
|
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| Abnova Taiwan Corp. | |||||||
| H00006271-Q01-25 |
S100A1 |
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Human | in vitro transl. |
25 µg /
€500.00
|
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| Abnova Taiwan Corp. | |||||||
Positive controls for S100A1 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-15647 |
S100A1 Lysate |
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0.1 mg /
€370.00
|
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for S100A1 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
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Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
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Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
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|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
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|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||


![Western Blot: S100A1 Lysate [NBL1-15647] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for S100A1](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/S100A1 Lysate-Western Blot-NBL1-15647-img0001.jpg)




