SP5355P S100A1 antibody

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0.1 mg / €370.00
Delivery Time: 7-10 working days

Quick Overview

Rabbit anti Bovine, Human, Mouse, Porcine, Rat S100A1

Product Description for S100A1

Rabbit anti Bovine, Human, Mouse, Porcine, Rat S100A1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for S100A1

Product Category Antibodies
Quantity 0.1 mg
Synonyms FocusOn041, S-100 protein alpha chain, S-100 protein subunit alpha, S100 alpha, S100 calcium-binding protein A1, S100-A1, S100A
Presentation Aff - Purified
Reactivity Bov, Hu, Ms, Por, Rt
Applications E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P23297
Immunogen:
Purified S100-alpha protein from Human pectoral muscle cells.
Genename:
6271
Antibody type Ab
Application ELISA: Assay dependent.
Immunoprecipitation (5 µg/ml).
Immunohistochemistry: Formalin-fixed, Paraffin-Embedded Sections (1 µg/ml): Demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland.
Western blotting (0.1 µg/ml): Detects a 10 kDa protein representing S100-alpha from Human muscle. S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
Background The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells.
Product Citations
  • Boerries M, Most P, Gledhill JR, Walker JE, Katus HA, Koch WJ, et al. Ca2+ -dependent interaction of S100A1 with F1-ATPase leads to an increased ATP content in cardiomyocytes. Mol Cell Biol. 2007 Jun;27(12):4365-73. Epub 2007 Apr 16. PubMed PMID: 17438143.
  • Pleger ST, Shan C, Ksienzyk J, Bekeredjian R, Boekstegers P, Hinkel R, et al. Cardiac AAV9-S100A1 gene therapy rescues post-ischemic heart failure in a preclinical large animal model. Sci Transl Med. 2011 Jul 20;3(92):92ra64. doi: 10.1126/scitranslmed.3002097. PubMed PMID: 21775667.
General Readings
  • Biomed. Res., 8(2): 119-125, 1987.
  • Neurochem. Int., 6(4): 513-518, 1984.
  • Mol Cell Proteomics. 2008 Mar;7(3):519-33.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Epitope Affinity Chromatography.
Buffer System:
PBS containing 15 mg/ml BSA as Stabilizer and 0.05% Sodium Azide as preservative.
State:
Lyophilized purified IgG fraction.
Aff - Purified
Reconstitution:
Reconstitute in 0.1 ml of sterile distilled water.
Species Reactivity
Species reactivity (tested):
Bovine, Human, Mouse, Porcine, and Rat.
Specificity
Specificity:
SP5355P detects S100-alpha protein and shows no cross-reactivity with the S100-beta protein.
Gene ID 6271
FocusOn and Reviews
FocusOns:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for S100A1 (3 products)

Catalog No. Species Pres. Purity   Source  
H00006271-P01-02

S100A1

S100A1 Human in vitro transl.
2 µg / €250.00
  Abnova Taiwan Corp.
H00006271-Q01-10

S100A1

S100A1 Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00006271-Q01-25

S100A1

S100A1 Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for S100A1 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-15647

S100A1 Lysate

Western Blot: S100A1 Lysate [NBL1-15647] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for S100A1
0.1 mg / €370.00
  Novus Biologicals Inc.