AP16925PU-N S100A4 (C-term) antibody

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0.1 mg / €270.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Goat anti Canine, Human, Mouse, Rat S100A4

AP16925PU-N

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  • AP16925PU-N

Product Description for S100A4

Goat anti Canine, Human, Mouse, Rat S100A4.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for S100A4

Product Category Antibodies
Target Category
Quantity 0.1 mg
Synonyms CAPL, Calvasculin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1, MTS1, Metastasin, Placental Calcium-Binding Protein, S100 Calcium-Binding Protein A4, S100-A4
Presentation Aff - Purified
Reactivity Can, Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Goat
Molecular weight 11.7 kDa (NP_002952.1 and NP_062427.1).
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P26447
Immunogen:
Synthetic peptide from the C-terminus of Human S100A4 (NP_002952.1 and NP_062427.1)
AA Sequence:
CNEFFEGFPDKQP
Genename:
6275
Remarks:
Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Property C-term
Antibody type Ab
Application Peptide ELISA: 1/128,000 (Detection Limit).
Western blot: 0.03-0.1 µg/ml. Approx 13 kDa band observed in Human placenta lysates.
Immunohistochemistry on Paraffin Sections: 2-3 µg/ml. 
In paraffin-embedded human small intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells.
Background S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.
Concentration 0.5 mg/ml
General Readings
  1. Li ZH, Bresnick AR. The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA. Cancer Res. 2006 May 15;66(10):5173-80. PubMed PMID: 16707441.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse, Rat, Canine (Dog).
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes S100A4 / CAPL.
Gene ID 6275
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for S100A4 (6 products)

Catalog No. Species Pres. Purity   Source  
AR09200PU-L

S100A4 (1-101, His-tag)

S100A4, 1-101 aa human, His-tagged, recombinant: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH
AR09200PU-N

S100A4 (1-101, His-tag)

S100A4, 1-101 aa human, His-tagged, recombinant: 15% SDS-PAGE (3 µg) Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH
NBC1-27983

S100A4

SDS-Page: S100A4 Protein [NBC1-27983] - S100A4, 13.8 kDa (121aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
0.1 mg / €340.00
  Novus Biologicals Inc.
GTX109153-pro

S100A4

S100A4 Purified Purified by conventional chromatography.
25 µg / €330.00
  GeneTex Inc.
H00006275-P01-10

S100A4

S100A4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00006275-P01-25

S100A4

S100A4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for S100A4 (2 products)

Catalog No. Species Pres. Purity   Source  
H00006275-T01

Metastasin Lysate(Denatured)

Metastasin Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-15656

S100A4 Lysate

Western Blot: S100A4 Lysate [NBL1-15656] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for S100A4
0.1 mg / €370.00
  Novus Biologicals Inc.
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