SQSTM1 (C-term) - AP20066PU-N | acris-antibodies.com

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Home » Antibodies » SQSTM1

Product Description for SQSTM1

Rabbit anti Human, Mouse, Rat SQSTM1.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for SQSTM1

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	EBI3-associated protein of 60 kDa, EBIAP, ORCA, OSIL, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Sequestosome-1, Ubiquitin-binding protein p62, p60, p62
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	E, P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: Q13501 Immunogen: SQSTM1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of Human SQSTM1. Genename: 8878
Property	C-term
Antibody type	Ab
Control peptide	AP20066CP-N
Application	ELISA. Western blot: SQSTM1 antibody can be used for the detection of SQSTM1 at 1–2 μg/ml. Positive Control: Human Spleen Tissue Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Spleen Tissue Lysate.
Background	SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget’s disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
Concentration	1.0 mg/ml
General Readings	Seibenhener ML, Babu JR, Geetha T, Wong HC, Krishna NR, Wooten MW. Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation. Mol Cell Biol. 2004 Sep;24(18):8055-68. PubMed PMID: 15340068. Hocking LJ, Lucas GJ, Daroszewska A, Mangion J, Olavesen M, Cundy T, et al. Domain-specific mutations in sequestosome 1 (SQSTM1) cause familial and sporadic Paget's disease. Hum Mol Genet. 2002 Oct 15;11(22):2735-9. PubMed PMID: 12374763. Rousiere M, Michou L, Cornelis F, et al. Paget’s disease of bone. Best Pract. Res. Clin. Rheumatol. 2003; 17:1019-41. Layfield R, Ciani B, Ralston SH, Hocking LJ, Sheppard PW, Searle MS, et al. Structural and functional studies of mutations affecting the UBA domain of SQSTM1 (p62) which cause Paget's disease of bone. Biochem Soc Trans. 2004 Nov;32(Pt 5):728-30. PubMed PMID: 15493999.
Storage	Upon receipt store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Immunoaffinity Chromatography Buffer System: PBS containing 0.02% Sodium Azide as preservative State: Liquid purified IgG fraction Aff - Purified
Species Reactivity	Species reactivity (tested): Human, Mouse and Rat
Gene ID	8878
FocusOn and Reviews	FocusOns: Autophagy - Recycling as a Survival Factor - FocusOn128
Targets	SQSTM1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for SQSTM1 (5 products)

Catalog No.	Species	Pres.	Purity		Source
AR09432PU-L	SQSTM1 (1-356, His-tag)
	Human	Purified	> 90 %		E. coli		0.5 mg / €670.00
							Acris Antibodies GmbH
AR09432PU-N	SQSTM1 (1-356, His-tag)
	Human	Purified	> 90 %		E. coli		0.1 mg / €250.00
							Acris Antibodies GmbH
NBP1-44490	SQSTM1
	Human	Purified					0.1 mg / €310.00
							Novus Biologicals Inc.
H00008878-P01-10	SQSTM1
	Human				in vitro transl.		10 µg / €350.00
							Abnova Taiwan Corp.
H00008878-P01-25	SQSTM1
	Human				in vitro transl.		25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for SQSTM1 (2 products)

Catalog No.	Species	Pres.	Purity		Source
NBL1-16446	sequestosome 1 Lysate
							0.1 mg / €370.00
							Novus Biologicals Inc.
H00008878-T01	SQSTM1 293T Cell Transient Overexpression Lysate(Denatured)
							0.1 ml / €240.00
							Abnova Taiwan Corp.

Recommended Secondary Antibodies for SQSTM1 (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

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