STAT3 pSer727 (incl. pos. control) - AM00149PU-N

Product Description for STAT3

Mouse anti Canine, Human, Mouse, Rat STAT3 23G5.
Properties: pSer727 (incl. pos. control)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for STAT3

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	APRF, Acute-phase response factor, Review 007, STAT-3, Signal transducer and activator of transcription 3
Presentation	Purified
Reactivity	Can, Hu, Ms, Rt
Applications	C, E, WB
Clonality	Monoclonal
Clone	23G5
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P40763 Immunogen: Synthetic phosphopeptide conjuagted to KLH Genename: 6774
Property	pSer727
Antibody type	Ab
Application	Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunohistochemistry.
Background	The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. Besides tyrosine phosphorylation, STAT3 activity is regulated by serine phosphorylation at serine 727. Recent reports indicate that both MAP kinase and SAP kinase induce phosphorylaton at serine 727.
Storage	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Shelf life: one year from despatch.
Format	Purification: Size exclusion chromatography Buffer System: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose State: Lyophilized purified IgG Purified Reconstitution: Restore with 1 ml H2O (15 min, RT).
Specificity	Specificity: This antibody specifically recognizes STAT3 phosphorylated at Ser 727. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated serine-phosphorylated proteins. Species: Human, Mouse, Rat, Dog.
Gene ID	6774
FocusOn and Reviews	Reviews: Antibody Panel to STAT Transcription Factors - Product Review 07
Targets	STAT3 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for STAT3 (2 products)

Catalog No.	Species	Source
H00006774-P01-10	STAT3
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00006774-P01-25	STAT3
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for STAT3 (2 products)

Catalog No.	Species
NBL1-16525	STAT3 Lysate
		0.1 mg / €500.00
		Novus Biologicals Inc.
NBL1-16526	STAT3 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for STAT3 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
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R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
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R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
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R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
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R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
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R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
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R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
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R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
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