Product Description for STAT3
Goat anti Human, Mouse, Rat STAT3.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Enzyme Immunoassay, Western blot / Immunoblot.
Properties for STAT3
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | APRF, Acute-phase response factor, Review 007, STAT-3, Signal transducer and activator of transcription 3 |
| Presentation | Aff - Purified |
| Reactivity | Hu, Ms, Rt |
| Applications | E, ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | immunohistochemistry, Peptide ELISA, Western Blot, This antibody is useful for Peptide ELISA Western Blot and Immunohistochemistry. Approx. 90kDa band observed in lysates of NIH3T3 (calculated MW of 88.0kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Stains mouse thymic medulla and not the cortex. , Peptide ELISA 1:128000, immunohistochemistry 10ug/ml, Western Blot 1-3 ug/ml |
| Background | STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage. STAT1 and STAT2 are induced by IFN alpha and forn1 a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4. |
| Concentration | 0.5 mg/ml |
| General Readings | Bromberg JF et al. Stat3 as an oncogene. Cell 98:295-303 (1999). |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Format |
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| Specificity |
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| Gene ID | 6774 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for STAT3 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00006774-P01-10 |
STAT3 |
||||||
 |
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00006774-P01-25 |
STAT3 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for STAT3 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-16525 |
STAT3 Lysate |
||||||
![Western Blot: STAT3 Lysate [NBL1-16525] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for STAT3](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/STAT3 Lysate-Western Blot-NBL1-16525-img0001.jpg) |
0.1 mg /
€500.00
|
||||||
| Novus Biologicals Inc. | |||||||
| NBL1-16526 |
STAT3 Lysate |
||||||
![Western Blot: STAT3 Lysate [NBL1-16526] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for STAT3](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/STAT3 Lysate-Western Blot-NBL1-16526-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for STAT3 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1317AP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1317B |
Goat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1317F |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1317HRP |
Goat IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1317T |
Goat IgG (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1416R |
Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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