AP12721PU-N STAT5 / STAT5A pSer726 antibody

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0.1 mg / €330.00
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Rabbit anti Human STAT5 / STAT5A

AP12721PU-N

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  • AP12721PU-N

Product Description for STAT5 / STAT5A

Rabbit anti Human STAT5 / STAT5A.
Properties: pSer726
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for STAT5 / STAT5A

Product Category Antibodies
Quantity 0.1 mg
Synonyms MGF, Mammary gland factor, Review007, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P42229
Immunogen:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser726 of human STAT5a.
Genename:
6776
Property pSer726
Antibody type Ab
Control peptide AP12721CP-N
Add. information Molecular weight: 90647 Da
Application ELISA: 1/1,000.
Western blot: 1/100-1/500.
Immunohistochemistry: 1/50-1/100.
Dot Blot: 1/500.
Background STAT5a is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this protein is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells.
Caution This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Concentration 0.25 mg/ml
General Readings
  • Martens, N., et al., J. Biol. Chem. 280(14):13817-13823 (2005).
  • Defilippi, P., et al., J. Cell Biol. 168(7):1099-1108 (2005).
  • Sekine, Y., et al., J. Biol. Chem. 280(9):8188-8196 (2005).
  • Sultan, A.S., et al., Oncogene 24(5):746-760 (2005).
  • Moriggl, R., et al., Cancer Cell 7(1):87-99 (2005).
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Buffer System:
PBS with 0.09% (W/V) Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects STAT5a pSer726
Species:
Human.
Gene ID 6776
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for STAT5 / STAT5A (5 products)

Catalog No. Species Pres. Purity   Source  
GTX28003

STAT5 / STAT5A

STAT5 / STAT5A
0.1 mg / €230.00
  GeneTex Inc.
H00006776-P01-10

STAT5 / STAT5A

STAT5 / STAT5A Human Purified
10 µg / €350.00
  Abnova Taiwan Corp.
H00006776-P01-25

STAT5 / STAT5A

STAT5 / STAT5A Human Purified
25 µg / €500.00
  Abnova Taiwan Corp.
H00006776-Q01-10

STAT5 / STAT5A

STAT5 / STAT5A Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00006776-Q01-25

STAT5 / STAT5A

STAT5 / STAT5A Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for STAT5 / STAT5A (2 products)

Catalog No. Species Pres. Purity   Source  
H00006776-T01

STAT5A 293T Cell Transient Overexpression Lysate(Denatured)

STAT5A 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €240.00
  Abnova Taiwan Corp.
NBL1-16528

STAT5A Lysate

Western Blot: STAT5A Lysate [NBL1-16528] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for STAT5A
0.1 mg / €370.00
  Novus Biologicals Inc.