Product Description for TIMP1
Rabbit anti Human TIMP1.
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.
Properties for TIMP1
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | CLGI, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, Review 002, TIMP, TIMP-1, Tissue inhibitor of metalloproteinases |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | Western Blot: 2-4 µg/ml for 2hrs at RT. Immunohistochemistry on Paraffin Sections: 1/100 for 10min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Positive Control: Conditioned, serum-free medium from TPA-treated human fetal lung cell line HFL-1. Breast, bladder, and ovarian carcinoma. |
| Background | Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of the matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 7076 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for TIMP1 (4 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09487PU-N |
TIMP1 |
||||||
 |
Human | > 95 % | E. coli |
10 µg /
€230.00
|
|||
| Acris Antibodies GmbH | |||||||
| AR09487PU-S |
TIMP1 |
||||||
|
Human | > 95 % | E. coli |
2 µg /
€130.00
|
|||
| Acris Antibodies GmbH | |||||||
| H00007076-P01-10 |
TIMP1 |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00007076-P01-25 |
TIMP1 |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for TIMP1 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-16924 |
TIMP1 Lysate |
||||||
![Western Blot: TIMP1 Lysate [NBL1-16924] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TIMP1.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/TIMP1 Lysate-Western Blot-NBL1-16924-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for TIMP1 (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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