TIMP1

Product Description for TIMP1

Mouse anti Human, Mouse, Rabbit, Rat TIMP1 3A3.
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for TIMP1

Product Category	Antibodies
Quantity	0.2 ml
Synonyms	CLGI, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, Review 002, TIMP, TIMP-1, Tissue inhibitor of metalloproteinases
Reactivity	Hu, Ms, Rb, Rt
Applications	P, WB
Clonality	Monoclonal
Clone	3A3
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P01033 Immunogen: The antibody reacts with the TIMP-1 antigen., Genename: 7076
Antibody type	Ab
Add. information	,
Application	The 3A4 monoclonal antibody has been tested for use in Western blotting and immunohistochemical analysis of formalin-fixed/paraffin-embedded tissues. It is suggested to use colon carcinoma as positive control. Antigen retrieval by microwave treatment (5-2, Western Blot.
Background	Tissue inhibitor of metalloproteinase 1 (TIMP-1) is a stromal factor which forms complexes with various members of matrix metalloproteinases (MMPs) and irreversibly inactivates them. A multifunctional glycoprotein, TIMP-1 is one of the four members of TIMP family (TIMP 1-4) which exhibits 50% sequence similarity and expressed in wide variety of cells (1). TIMP-1 binds non-covalently in a 1:1 stoichiometric fashion to the active form of MMPs thereby inhibiting the proteolytic activity of these enzymes. Including inhibitory function, other studies have proposed growth protion, anti-apoptotic, anti- and pro-angiogenic functions for TIMP-1. TIMP-1 expression has been observed in poor clinical outcome in many cancer types, making it ideal prognostic marker in various cancer (2).
General Readings	,
Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format	Purification: IgG purified
Specificity	Specificity: This monoclonal antibody binds to human TIMP-2. Reactivity in other species has not been determined, but the 11-mer peptide used for the immunization shows a 100% match with rat, mouse, and rabbit TIMP-2. The antibody was tested for cross-reactivity with TIMP-1 and did not cross-react. The 3A4 monoclonal antibody was generated from mice immunized with an 11 mer-peptide derived from the sequence of human TIMP-2. The amino acid sequence of this peptide was: DSGNDIYGNPI (Murray et al., 1998)., Species: Human, Mouse, Rat
Gene ID	7076
FocusOn and Reviews	Reviews: Antibodies to TIMP Protein Family - Product Review 02
Targets	TIMP1 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for TIMP1 (4 products)

Catalog No.	Species	Purity	Source
AR09487PU-N	TIMP1
	Human	> 95 %	E. coli	10 µg / €230.00
				Acris Antibodies GmbH
AR09487PU-S	TIMP1
	Human	> 95 %	E. coli	2 µg / €130.00
				Acris Antibodies GmbH
H00007076-P01-10	TIMP1
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00007076-P01-25	TIMP1
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.

Positive controls for TIMP1 (1 products)

Catalog No.	Species
NBL1-16924	TIMP1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for TIMP1 (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH