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Product Description for ATF2
Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, cAMP response element-binding protein CRE-BP1, Cyclic AMP-responsive element-binding protein 2
Properties for ATF2
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 100 µg |
| Reactivity | Human, Mouse |
| Presentation | Aff - Purified |
| Applications | Paraffin Sections, Western blot / Immunoblot |
| Host | Rabbit |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | ATF-2, CREB2, CREBP1, Activating transcription factor 2, CRE-BP1, HB16, CREB-2, Review 018 |
| Swiss Prot Num | P15336 |
| Immunogen | The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 62 or 44 (N-D-SP-V-I). |
| Product Type | Antibodies |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465. 2. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181(2): 629-635. 3. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. 4. Gupta S, et al. (1995) Science. 267: 389-393. 5. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811. |
| Format | State: Liquid purified IgG |
| Purification: Affinity-chromatography using epitope-specific immunogen | |
| Buffer System: PBS(without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol | |
| Specificity | ATF-2 antibody detects endogenous levels of total ATF-2 protein. |
| Application | Western Blot: 1:500~1:1000. Immunohistochemistry: 1:50~1:100. |
| Background | ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain. |
| Concentration | 1.0 mg/ml |
| Review | r018 Antibody Panel to CRE Binding Proteins |
Secondary Antibodies for ATF2
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
