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Product Description for Actin (pan)
Properties for Actin (pan)
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 ml |
| Reactivity | Human, Mouse, Rat |
| Presentation | Aff - Purified |
| Applications | Frozen sections, Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot, Immunoprecipitation |
| Host | Rabbit |
| Clone | S12-I |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Review 008 |
| Immunogen | Peptide derived from C-terminal sequence of human β-actin. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format | State: Liquid purified Ig fraction. |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 20 mM Tris-HCl, pH 8.0 containing 0.05% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer. Note: This antibody is aslo available without Stabilizer. | |
| Specificity | This antibody recognises also the alpha-Actin. |
| Application | Immunohistochemistry (Frozen Sections, Fixed, Paraffin-Embedded Sections). Immunoprecipitation. Western blot (1/2,000): Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100 Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. ELISA (1/10,000-1/20,000). |
| Background | The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in nearly all cells. There are three a-actins (a-skeletal, a-cardiac and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. The actin in cells of various species and tissues are very similar in their immunological and physical properties. As a consequence, it is difficult to produce antisera to this protein. The availability of monoclonal antibody to actin provides a specific and useful tool in studying actin structure and function and in probing binding sites of actin-binding proteins. |
| Concentration | 1.9 mg/ml |
| Review | r008 Antibody Panel to Actin |
Secondary Antibodies for Actin (pan)
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
