CD11b

Product Description for CD11b

Mouse anti Human CD11b F6.2.
Presentation: PE
Product is tested for Flow Cytometry.

Properties for CD11b

Product Category	Antibodies
Quantity	100 Tests
Synonyms	CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Presentation	PE
Reactivity	Hu
Applications	F
Clonality	Monoclonal
Clone	F6.2
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P11215 Genename: 3684
Antibody type	Ab
Application	Flow Cytometry. Monitoring of subsets in peripheral blood. Characterization of leukemias and lymphomas. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Background	(Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes.
Concentration	Lot specific
Protocols	PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of MAB/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
General Readings	Arnaout MA. Structure and function of the leukocyte adhesion molecules CD11/CD18. Blood. 1990 Mar 1;75(5):1037-50. PubMed PMID: 1968349. Leukocyte adhesion to endothelial cells. Wautier, ,J.L., Sediadi, H., Vilette, d., Weill, D., Wautier, M.P.. Biorheology . 1990:27(3-4): 425-32. Babcock GF, Alexander JW, Warden GD. Flow cytometric analysis of neutrophil subsets in thermally injured patients developing infection. Clin Immunol Immunopathol. 1990 Jan;54(1):117-25. PubMed PMID: 1967160. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med. 1987;38:175. Merrill JT, Slade SG, Weissmann G, Winchester R, Buyon JP. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation. J Immunol. 1990 Oct 15;145(8):2608-15. PubMed PMID: 1976698. Richter J, Ng-Sikorski J, Olsson I, Andersson T. Tumor necrosis factor-induced degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin-triggered oscillations of cytosolic free Ca2+. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9472-6. PubMed PMID: 1979172.
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Format	Label: PE Buffer System: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative. Purity: PE State: Liquid purified IgG fraction.
Specificity	Species: Human.
Gene ID	3684
Targets	CD11b antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for CD11b (4 products)

Catalog No.	Species	Pres.	Source
H00003684-Q01-10	CD11b
	Human		in vitro transl.	10 µg / €350.00
				Abnova Taiwan Corp.
H00003684-Q01-25	CD11b
	Human		in vitro transl.	25 µg / €500.00
				Abnova Taiwan Corp.
PR27036-2	CD11b
	Human	Purified	E. coli	2 µg / €330.00
				Neuromics Antibodies
PR27036-5	CD11b
	Human	Purified	E. coli	5 µg / €450.00
				Neuromics Antibodies

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