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Product Description for Ki-67
Properties for Ki-67
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.5 ml |
| Reactivity | Human, Rat |
| Presentation | Supernatant |
| Applications | Paraffin Sections, Western blot / Immunoblot |
| Isotype | IgG |
| Host | Rabbit |
| Clone | SP6 |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Ki67, MKI67, FocusOn056 |
| Swiss Prot Num | P46013 |
| Immunogen | A synthetic peptide from C-terminus of human Ki-67. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format | State: Liquid Tissue Culture Supernatant containing Sodium Azide as preservative. |
| Specificity | Reacts with Ki-67. Cellular localization: Nuclear. |
| Application | Immunohistochemistry: Use Ki67 antibody at a 1/100 dilution in an ABC method for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Also suitable for Western blot. |
| Background | Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation. |
| Species Reactivity | Tested: Human and Rat. |
| Focuson | 056 Proliferation Marker Antibodies - FocusOn No.56 |
Secondary Antibodies for Ki-67
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
