Antibodies to Ubiquitin

Introduction: Ubiquitin regulates the protein turnover in a cell.

Ubiquitin is a small, 76-residue protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein\'s function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction.

Ubiquitin+1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially ub+1 is accumulated in aggregates containing amyloid-beta and phosphorylated-tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer\'s disease. Also ub +1 acts as an aggravating factor in polyglutamine induced neurodegeneration.

Antibody Tools for the Detection of Ubiquitin

Acris Antibodies offers a wide range of antibodies for the detection of Ubiquitin. These antibodies are available in various formats and can be used in immunological methods like Western blotting, ELISA or immunohistochemistry.

Fig.1: Immunohistochemical staining of Ubiquitin in Formalin fixed, Paraffin processed tissue of Human hypophysis using antibody SM1422P

Fig.2: Immunoblot of Ubiquitin using antibody R1199: Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by immunoblot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche). Other detection systems will yield similar results

Fig.3: Immunohistochemical staining of Ubiquitin in hippocampal tissue from an Alzheimer patient, using antibody NB 300-130.

Fig.4: Blot using Anti-Polyubiquitin Chain (antibody MC-034), which reacts with the iso-binding site (between Ubiquitin and Ubiquitin). MW markers: 103 kDa, 77 kDa, 50 kDa, 34.3 kDa, 28.8 kDa and 20.7 kDa.

Fig.5: Immunoblots of 2 ng recombinant human Ubiquitin and 2, 1, and 0.5 ng recombinant human Ubiquitin+1. Samples were electrophoresed on 15% gels and immunoblotting was with 1.0 µg/mL anti-Ubiquitin+1 (antibody RA15043). A one minute exposure is shown.

Available Antibody Panel to Ubiquitin

Ubiquitin antibody, Ubiquitin+1, Polyubiquitin Chain, Ubiquitin-conjugating enzyme E2N (UBC13).

 

147 related products - click here to filter product result list

Antibodies

Catalog No.	Clone/Isotype		Presentation	Reactivity	Applications
NB110-55671	Cbl antibody
	YE315		Purified	Hu, Rt	WB, F, IF	0.1 ml / 380 €
	Rabbit	IgG	Novus Biologicals Inc.
NB110-55672	Cbl antibody
	YE323		Purified	Hu	IP, WB, F	0.1 ml / 380 €
	Rabbit	IgG	Novus Biologicals Inc.
NB100-78591	CBL Antibody
			Purified	Hu	WB	0.1 mg / 370 €
	Rabbit		Novus Biologicals Inc.
10964-2-AP	CDC34 antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB100-78600	CYLD Antibody
			Aff - Purified	Hu	WB	0.1 mg / 370 €
	Rabbit		Novus Biologicals Inc.
10813-1-AP	HERPUD1 antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10105-2-AP	HSPC150 antibody
			Aff - Purified	Hu	E, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB100-2220B	LC3 Antibody
			Aff - Purified	Hu, Ms, Rt	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB100-2220H	LC3 Antibody
			Aff - Purified	Hu, Ms, Rt	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB100-2220L	LC3 Antibody
			Aff - Purified	Hu, Ms, Rt	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB100-2331B	LC3 Antibody
			Aff - Purified	Hu, Ms	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB100-2331H	LC3 Antibody
			Aff - Purified	Hu, Ms	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB100-2331L	LC3 Antibody
			Aff - Purified	Hu, Ms	C, IP, P, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB600-1384B	LC3B Antibody
			Serum	Hu, Ms	C, IF, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB600-1384H	LC3B Antibody
			Serum	Hu, Ms	C, IF, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB600-1384L	LC3B Antibody
			Serum	Hu, Ms	C, IF, WB	0.1 ml / 440 €
	Rabbit		Novus Biologicals Inc.
NB110-57179	MAPILC3A (C-term) antibody
	EP1983Y		Aff - Purified	Hu, Ms, Rt	IP, WB, F, IF	0.1 ml / 380 €
	Rabbit	IgG	Novus Biologicals Inc.
10573-1-AP	OTB1 antibody
			Aff - Purified	Hu	E, P	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB110-55673	Phospho-Cbl (pY774) antibody
	E160		Purified	Hu	IP, WB, F, IF	0.1 ml / 380 €
	Rabbit	IgG	Novus Biologicals Inc.
NB110-57157	Phospho-LAT (pY191) antibody
	E225		Aff - Purified	Hu, Ms, Rt	IP, WB, F, IF	0.1 ml / 380 €
	Rabbit	IgG	Novus Biologicals Inc.
MC034	Polyubiquitin Chain (iso-peptide binding site) antibody
	21A8		Purified	Rb	E, WB	0.1 mg / 660 €
	Mouse	IgM	Kamiya Biomedical Company
AM00587SU-N	Protein Gene Product 9.5 (PGP9.5) antibody
	10A1		Supernatant	Hu	P	0.5 ml / 330 €
	Mouse	IgG2b	Acris Antibodies GmbH
BM160	Protein Gene Product 9.5 (PGP9.5) antibody
	13C4		Purified	Hu, Rt, GP	E, WB, P	0.2 ml / 350 €
	Mouse	IgG2a	Acris Antibodies GmbH
BM699	Protein Gene Product 9.5 (PGP9.5) antibody
	31A3		Purified	Hu, Rt	C, P, E, WB	0.2 ml / 370 €
	Mouse	IgG1	Acris Antibodies GmbH
BP170	Protein Gene Product 9.5 (PGP9.5) antibody
	-		Serum	Mam, Hu	P, WB	50 µl / 310 €
	Rabbit		Acris Antibodies GmbH
SM1673P	Protein Gene Product 9.5 (PGP9.5) antibody
	10A1		Supernatant	Hu	C, P, WB	1 ml / 530 €
	Mouse	IgG2b	Acris Antibodies GmbH
SP1418	Protein Gene Product 9.5 (PGP9.5) antibody
	-		Serum	Hu	WB	0.1 ml / 350 €
	Goat		Acris Antibodies GmbH
MO20002-100	Protein Gene Product 9.5 (PGP9.5) antibody
	10A1		Supernatant	Rt, Ms, Hu	C, P, WB	0.1 ml / 440 €
	Mouse	IgG2b	Neuromics Antibodies
NB110-58869	Protein Gene Product 9.5 (PGP9.5) antibody
	BH7		Supernatant	Bov, Hu, Rt	WB, IF	0.5 ml / 300 €
	Mouse	IgG1	Novus Biologicals Inc.
NB110-58872	Protein Gene Product 9.5 (PGP9.5) antibody
			Purified	Hu	WB, IF	0.1 ml / 300 €
	Chicken		Novus Biologicals Inc.
NB110-58874	Protein Gene Product 9.5 (PGP9.5) antibody
			Serum	Hu, Rt	WB, IF	0.1 ml / 300 €
	Rabbit		Novus Biologicals Inc.
NB300-675	Protein Gene Product 9.5 (PGP9.5) antibody
			Aff - Purified	Ms	WB	0.1 ml / 290 €
	Rabbit		Novus Biologicals Inc.
NB300-676	Protein Gene Product 9.5 (PGP9.5) antibody
			Aff - Purified	Ms	WB	0.1 ml / 290 €
	Rabbit		Novus Biologicals Inc.
GP14104-150	Protein Gene Product 9.5 (PGP9.5) (175-191) antibody
	-		Serum	Hu, Rt, Ms	C	0.15 ml / 430 €
	Guinea Pig		Neuromics Antibodies
GP14104-50	Protein Gene Product 9.5 (PGP9.5) (175-191) antibody
	-		Serum	Hu, Rt, Ms	C	50 µl / 250 €
	Guinea Pig		Neuromics Antibodies
RA12103-150	Protein Gene Product 9.5 (PGP9.5) (175-191) antibody
	-		Serum	Hu, Rt, Ms, Por	C	0.15 ml / 360 €
	Rabbit		Neuromics Antibodies
RA12103-50	Protein Gene Product 9.5 (PGP9.5) (175-191) antibody
	-		Serum	Hu, Rt, Ms, Por	C	50 µl / 180 €
	Rabbit		Neuromics Antibodies
P10135	Protein Gene Product 9.5 (PGP9.5) (175-191) control peptide antibody
	-		Purified			20 µg / 120 €
			Neuromics Antibodies
10229-1-AP	SAE1 antibody
			Aff - Purified	Hu, Ms	E, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB100-57095	UBASH3A antibody
			Aff - Purified		E	0.1 mg / 250 €
	Goat	IgG	Novus Biologicals Inc.
10201-2-AP	UBB antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10457-1-AP	UBC antibody
			Aff - Purified	Hu, Ms	E, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB100-41432	UBCH7 antibody
			Aff - Purified	Hu		0.1 ml / 400 €
	Rabbit		Novus Biologicals Inc.
NB100-58804	UBE1 antibody
			Aff - Purified	Hu, Ms	IP, WB	0.02 mg / 380 €
	Rabbit		Novus Biologicals Inc.
NB100-58805	UBE1 antibody
			Aff - Purified	Hu, Ms	IP, WB	0.02 mg / 380 €
	Rabbit		Novus Biologicals Inc.
NB100-58806	UBE1 antibody
			Aff - Purified	Hu	IP	0.1 mg / 380 €
	Rabbit		Novus Biologicals Inc.
NB100-58807	UBE1 antibody
			Aff - Purified	Hu, Ms	WB	0.02 mg / 380 €
	Rabbit		Novus Biologicals Inc.
EB08151	UBE1L2 antibody
	-		Aff - Purified			0.1 mg / 200 €
	Goat		Everest Biotech Ltd.
10733-1-AP	UBE2B antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
11373-1-AP	UBE2D1 antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10722-1-AP	UBE2G2 antibody
			Aff - Purified	Hu	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10070-1-AP	UBE2I antibody
			Aff - Purified	Hu	E, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10224-1-AP	UBE2I antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
51018-2-AP	UBE2I antibody
			Aff - Purified	Hu, Ms, Rt	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10243-1-AP	UBE2N antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
ARP34089_T100	UBE2N (ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)) antibody
			Purified	Hu	WB	0.1 mg / 240 €
	Rabbit	IgG	AVIVA Systems Biology
10207-2-AP	UBE2V1 antibody
			Aff - Purified	Hu	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10689-1-AP	UBE2V2 antibody
			Aff - Purified	Hu, Ms	E, P, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB100-58802	UBE4B antibody
			Aff - Purified	Hu, Ms	IP, WB	0.02 mg / 380 €
	Rabbit		Novus Biologicals Inc.
NB100-58803	UBE4B antibody
			Aff - Purified	Hu	IP	0.1 mg / 380 €
	Rabbit		Novus Biologicals Inc.
AP01102PU-N	Ubiquitin antibody
			Purified	Bov	WB, ID	1 ml / 160 €
	Rabbit		Acris Antibodies GmbH
BM758	Ubiquitin antibody
	242.9		Ascites	Hu	C, P, WB	0.1 ml / 590 €
	Mouse	IgG1	Acris Antibodies GmbH
BP875	Ubiquitin antibody
	-		Ig Fraction	Bov	E	1 ml / 220 €
	Sheep		Acris Antibodies GmbH
DM225	Ubiquitin antibody
	FPM1		Supernatant	Hu	C, P	1 ml / 300 €
	Mouse	IgG1	Acris Antibodies GmbH
DP040	Ubiquitin antibody
	-		Purified	Hu, Mam	C, P	1 ml / 360 €
	Rabbit		Acris Antibodies GmbH
DP040-05	Ubiquitin antibody
	-		Purified	Hu, Mam	C, P	0,5 ml / 220 €
	Rabbit		Acris Antibodies GmbH
R1199	Ubiquitin antibody
	-		Purified	Mam, Yeast	E, WB	0.5 mg / 340 €
	Rabbit		Acris Antibodies GmbH
SM1422P	Ubiquitin antibody
	1B4-UB		Purified	Hu	C, P, E, WB	0.1 mg / 380 €
	Mouse	IgG1	Acris Antibodies GmbH
15-288-10073F	Ubiquitin antibody
	-		Aff - Purified	Hu, Ms, Rt	E, WB	0.1 mg / 360 €
	Chicken		GenWay Biotech Inc.
15-288-10073F-50	Ubiquitin antibody
	-		Aff - Purified	Hu, Ms, Rt	E, WB	50 µg / 230 €
	Chicken		GenWay Biotech Inc.
MC033	Ubiquitin antibody
	KM691		Ascites	Hu	P, WB	0.1 ml / 350 €
	Mouse	IgM	Kamiya Biomedical Company
MC046	Ubiquitin antibody
	Ubi-1			Mam	IF, WB	0.1 ml / 380 €
	Mouse	IgG1	Kamiya Biomedical Company
PC208	Ubiquitin antibody
	-			Mam	IF, WB	0.1 ml / 380 €
	Rabbit		Kamiya Biomedical Company
LF-MA0118	Ubiquitin antibody
	2A1		Purified	Hu	E, IP, WB	0.1 mg / 330 €
	Mouse	IgG1	LabFrontier Co. Ltd.
LF-MA0119	Ubiquitin antibody
	4A1		Purified	Hu	E, IP, WB	0.1 mg / 330 €
	Mouse	IgG1	LabFrontier Co. Ltd.
LF-PA0078	Ubiquitin antibody
	-		Purified	Hu	WB	0.1 mg / 330 €
	Rabbit		LabFrontier Co. Ltd.
MO18001-100	Ubiquitin antibody
	PD41		Purified	Hu, Ms, Rt	P, WB	0.1 ml / 380 €
	Mouse	IgG	Neuromics Antibodies
MO19005-100	Ubiquitin antibody
	-		Ascites	Hu	P, WB	0.1 ml / 310 €
	Mouse		Neuromics Antibodies
MO19005-50	Ubiquitin antibody
	-		Ascites	Hu	P, WB	50 µl / 220 €
	Mouse		Neuromics Antibodies
NB100-1650	Ubiquitin antibody
	10C2-2		Supernatant	Mam	IP, E, C, P, WB	1 ml / 410 €
	Mouse	IgG1	Novus Biologicals Inc.
NB300-129	Ubiquitin antibody
	-		Serum	Mam, Chk, Dros, C. elegans	C, P, E, IP, WB	50 µl / 300 €
	Rabbit		Novus Biologicals Inc.
NB300-130	Ubiquitin antibody
	Ubi-1		Ascites	Hu, Bov, Chk, Dros, C. elegans	IP, WB, C, P, E	50 µg / 300 €
	Mouse	IgG1	Novus Biologicals Inc.
NB300-262	Ubiquitin antibody
	-		Aff - Purified	Plant	WB	0.1 mg / 330 €
	Rabbit		Novus Biologicals Inc.
NB300-263	Ubiquitin antibody
	-		Aff - Purified	Mam	WB	0.1 mg / 330 €
	Rabbit		Novus Biologicals Inc.
NB600-473	Ubiquitin antibody
			Ig Fraction	Yeast	E, WB	0.5 mg / 410 €
	Rabbit		Novus Biologicals Inc.
NB600-776	Ubiquitin antibody
	1B4-UB		Ig Fraction	Hu, Bov	E, F, C, P, WB	50 µg / 330 €
	Mouse	IgG1	Novus Biologicals Inc.
11012	Ubiquitin antibody
	-		Purified	Hu	WB	1 mg / 350 €
	Chicken		QED Bioscience Inc.
56354-200	Ubiquitin antibody
	-		Serum	Mam, Chk, Fish, Xen, Yeast, Dros, E. coli	C, IP, WB	0.2 ml / 390 €
	Rabbit		QED Bioscience Inc.
56354-50	Ubiquitin antibody
	-		Serum	Mam, Chk, Fish, Xen, Yeast, Dros, E. coli	C, IP, WB	50 µl / 240 €
	Rabbit		QED Bioscience Inc.
15-288-22910	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) antibody
	-		Aff - Purified	Hu	WB	0.1 mg / 360 €
	Chicken		GenWay Biotech Inc.
15-288-22910-50	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) antibody
	-		Aff - Purified	Hu	WB	50 µg / 230 €
	Chicken		GenWay Biotech Inc.
AP00442PU-N	Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) (internal) antibody
	-		Aff - Purified	Hu, Ms, Rt	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP00443PU-N	Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) (internal) antibody
	-		Aff - Purified	Hu, Ms, Rt	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP00448PU-N	Ubiquitin specific protease 2 (USP2) (internal) antibody
	-		Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP01056PU-N	Ubiquitin specific protease 20 (USP20/VDU2) (internal) antibody
			Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP01057PU-N	Ubiquitin specific protease 20 (USP20/VDU2) (internal) antibody
			Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP01054PU-N	Ubiquitin specific protease 33 (USP33/VDU1) (internal) antibody
			Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP01055PU-N	Ubiquitin specific protease 33 (USP33/VDU1) (internal) antibody
			Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP00444PU-N	Ubiquitin Specific Protease 4 (USP4) (internal) antibody
	-		Aff - Purified	Hu	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
AP00445PU-N	Ubiquitin Specific Protease 4 (USP4) (internal) antibody
	-		Aff - Purified	Hu, Ms	P	50 µg / 380 €
	Rabbit		Acris Antibodies GmbH
LF-MA0087	Ubiquitin+1 antibody
	40B3		Purified	Hu	E, IP, WB	0.1 mg / 330 €
	Mouse	IgG1	LabFrontier Co. Ltd.
LF-PA0101	Ubiquitin+1 antibody
	-		Purified	Hu	IP, WB	0.1 mg / 330 €
	Rabbit		LabFrontier Co. Ltd.
MO15045-100	Ubiquitin+1 antibody
	-		Purified	Hu	WB	0.1 mg / 290 €
	Mouse	IgG2b	Neuromics Antibodies
RA15043-100	Ubiquitin+1 antibody
	-		Aff - Purified	Hu	C, P, WB	0.1 mg / 430 €
	Rabbit		Neuromics Antibodies
15-288-21294	Ubiquitin-conjugating enzyme E2 Q2 antibody
	-		Aff - Purified	Hu	WB	0.1 mg / 360 €
	Chicken		GenWay Biotech Inc.
15-288-21294-50	Ubiquitin-conjugating enzyme E2 Q2 antibody
	-		Aff - Purified	Hu	WB	50 µg / 230 €
	Chicken		GenWay Biotech Inc.
SP2158P	Ubiquitin-conjugating enzyme E2N (UBC13) antibody
	-		Aff - Purified	Hu, Ms, Rt	P, WB	0.1 mg / 260 €
	Rabbit		Acris Antibodies GmbH
NB100-80857	Ubiquitin-conjugating Enzyme E2N Antibody
			Aff - Purified	Hu, Ms, Rt	C, WB	0.05 mg / 410 €
	Rabbit		Novus Biologicals Inc.
10329-1-AP	UBL1 antibody
			Aff - Purified	Hu	E, WB	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
10715-1-AP	UBL5 antibody
			Aff - Purified	Hu	E	150 µl / 320 €
	Rabbit	IgG	ProteinTech Group, Inc.
NB110-55621	UCHL3 antibody
			Aff - Purified	Hu	IF	0.05 mg / 400 €
	Rabbit		Novus Biologicals Inc.
10615-1-AP	UFD1L antibody
			Aff - Purified	Hu, Ms