Antibodies to desArg9-Bradykinin and Bradykinin Receptors

Introduction

Bradykinins (BKs) are a family of short, structurally similar peptides that activate sensory fibres, contract venous smooth muscle, stimulate release of cytokines, induce connective tissue proliferation and mediate endothelium-dependent vasodilation. BK antagonists are of potential use in the treatment of inflammation, asthma, mild pain and endotoxic shock.

BK receptors are widespread in peripheral tissues, and at least 3 different receptor subtypes have been proposed. Of these, B2 is the predominant subtype, mediating slow contraction of various smooth muscles (including veins, intestine, uterus, trachea and lung), inducing endothelium-dependent relaxation of arteries and arterioles, and stimulating natriuresis/diuresis in kidney. Bradykinin B1 receptor is generally only minimally expressed under normal physiological conditions, but the expression is induced rapidly under pathologic conditions such as ischemia, atheromatous disease, or exposure to inflammatory cytokines. Expression has been reported in brain, colon, kidney, lung, nasal mucosa, pancreas, prostate, skin, spinal cord, and vessel. Des-Arg9-bradykinin, the active metabolite of bradykinin is the selective bradykinin B1-receptor agonist.

Antibody Panel to desArg9-Bradykinin and Bradykinin Receptors

Acris Antibodies offers a range of polyclonal antibodies to desArg9-Bradykinin, Bradykinin B1 and Bradykinin B2 Receptor .The antibodies can be used for immunohistochemistry on paraffin embedded sections and other applications, respectively . All antibodies are reactiveon human and rodent tissues. Receptor-antibodies were made to cytoplasmic domains to achieve maximum specifity .

Fig. 1: Immunohistochemical staining of smooth muscle (artery) using antibody SP4078P

Fig. 2: Immunohistochemical staining of nasal mucosa using antibody SP4076P

Antibody Panel to desArg9-Bradykinin and Bradykinin Receptors

desArg9-Bradykinin , Bradykinin B1 receptor, Bradykinin B2 receptor, antibody.

Literature

Gardes J, Michineau S, Pizard A, Alhenc-Gelas F, Rajerison RM. Aspirin inhibits human bradykinin B(2) receptor ligand binding function. Biochem Pharmacol. 2008 Feb 12 [Epub ahead of print]

Medeiros R, Cabrini DA, Ferreira J, Fernandes ES, Mori MA, Psquero JB, Bader M, Avellae MC, Campos MM, Calixto JB: Bradykinin B1 receptor expression induced by tissue damage in the rat portal vein:… Circ. Res. 2004 May 28; 94(10): 1375-82.

Zhang Y, Adner M, Cardell LO. Up-regulation of bradykinin receptors ina murine in-vitro model of chronic airway inflammation. Eur J Pharmacol. 2004 Apr. 5; 489(1-2): 117-26.

Pinheiro RM, Campos MM, Calixto JB. Analysis of the mechanisms involved in the inflammatory response induced by des-Arg9-bradykinin in the rat pleural cavity. Inflamm Res. 2001 Nov; 50(11): 570-6.