Antibodies to Cardiotin

Introduction

Cardiotin is a high molecular weight protein complex (300 kDa) expressed in the myocard of several species and to a lesser extent also in skeletal muscle. Cardiotin is not found in smooth muscle tissues, other mesenchymal or epithelial tissues.

The cardiotin distribution pattern is independent of other sarcomeric components, such as desmin, myosin, actin, titin, nebulin, and desmoplakin, and shows a longitudinal filamentous localization between myofibrils. The cardiotin structure exists of subunits of 60 kDa and 100 kDa containing both the same amino-terminal 14 amino-acid sequence. The tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myo¬cardium. Monoclonal cardiotin antibodies can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.

Antibody Panel to Cardiotin

Acris Antibodies offers a range of monoclonal IgM antibodies to Cardiotin suitable for Western blot and / or Immunohistochemical applications on frozen or paraffin embedded tissues.

Fig. 1: Human atrial cardiomyocytes stained with the monoclonal antibody R2G (BM6043P)

Fig. 2: Immunoblotting of the microsomal fraction of porcine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies : a)SR-2 (BM6044P) b)SR-3 (BM6045P) c)R2G (BM6043P)

Antibody Panel to Cardiotin

Cardiotin antibody.

Literature

Schaart G. et al. (1997) Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein. FEBS Lett. 1997 Feb 17;403(2):168-72.

Ausma J. et al. (2003) Reverse structural and gap-junctional remodelling after prolonged atrial fibrillation in the goat. Circultation. 2003 Apr 22;107(15):2051-8.

Schaart G, van der Ven PF, Ramaekers FC. Characterization of cardiotin, a structural component in the myocard. Eur J Cell Biol. 1993 Oct;62(1):34-48.